DNase, containing 3 tryptophan residues, can be inactivated by modification of this amino acid with Nbromosuccinimide. Chromatography of acid hydrolysates shows all three tryptophan residues modified by the time inactivation is complete. However, spectrophotometric measurement indicates that only 2 residues are modified. The discrepancy is due to the unreliability of the spectrophotometric meth...

Two titrimetric and two spectrophotometric methods are described for the assay of famotidine (FMT) in tablets using N-bromosuccinimide (NBS). The first titrimetric method is direct in which FMT is titrated directly with NBS in HCl medium using methyl orange as indicator (method A). The remaining three methods are indirect in which the unreacted NBS is determined after the complete reaction betw...

Conditions are described for the production of large amounts of an extracellular elastolytic protease by Vibrio vulnificus. The yield of enzyme was maximal during the late exponential growth phase and was stable during the stationary growth phase in a medium composed of 2% Proteose Peptone and 1.5% NaCl. The protease has a molecular weight of ca. 50,500 (estimated by sodium dodecyl sulfate-poly...

a copolymer of styrene and methyl styrene was synthesized by nitroxide - mediated “living” free radical polymerization )nmp). n-bromosuccinimide was used as a brominating agent to obtain a copolymer with bromine as its end group. this brominated copolymer was used as macro initiators for atom transfer  radical  polymerization  (atrp).  chemical  modifcation  of  the  clay  and transformation of...

The tryptophan residues of P nerve growth factor (J3NGF) were modified by reaction with varying concentrations of N-bromosuccinimide. Analysis of the oxidized derivatives by electrophoresis under denaturing conditions, before and after reduction or oxidation of disulfide bonds, revealed partial hydrolysis of specific peptide bonds. Analysis of the newly created NH2 termini by the 5-dimethylami...

HE PURPOSES OF THE PRESENT PAPER are to describe some new sensitive tests suitable for the detection of bile in biologic fluids which overcome the defects demonstrated in procedures described in most standard texts, and also to confirm the experiments of C. E. May et at. (1). The sensitivity of the proposed oxidation tests to detect bilirubin, viz. ferricyanide, molybdate, N-bromosuccinimide (2...