نتایج جستجو برای: multiplex rt

تعداد نتایج: 80305  

Journal: : 2023

Virus dịch tả lợn Châu Phi (ASFV) và virus cổ điển (CSFV) đã gây thiệt hại kinh tế rất lớn cho người chăn nuôi lợn. Có nhiều phương pháp xét nghiệm để phát hiện hai này như: PCR, Realtime phân lập virus, ELISA. Bên cạnh đó Multiplex Real-time RT-PCR (mReal-time RT-PCR) đồng thời ASFV CSFV cũng được đánh giá ở một số nước trên thế giới nhưng chưa nghiên cứu tại Việt Nam. Trong chúng tôi mReal-ti...

Journal: :Phytopathology 2010
R K Yokomi M Saponari P J Sieburth

A multiplex Taqman-based real-time reverse transcription (RT) polymerase chain reaction (PCR) assay was developed to identify potential severe strains of Citrus tristeza virus (CTV) and separate genotypes that react with the monoclonal antibody MCA13. Three strain-specific probes were developed using intergene sequences between the major and minor coat protein genes (CPi) in a multiplex reactio...

2012
Jingzi Piao Jun Jiang Bianli Xu Xiaohong Wang Yanfang Guan Weili Wu Licheng Liu Yuan Zhang Xueyong Huang Pengzhi Wang Jinyin Zhao Xiaoping Kang Hua Jiang Yuanyin Cao Yuling Zheng Yongqiang Jiang Yan Li Yinhui Yang Weijun Chen

Simultaneous detection of enteric viruses that cause similar symptoms (e.g. hand, foot and mouth disease) is essential to the prevention of outbreaks and control of infections. In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and used for simult...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2002
Abdul K Siraj Ugur Ozbek Sudha Sazawal Sema Sirma Georgina Timson Abdallah Al-Nasser Manorama Bhargava Hassan El Solh Kishor Bhatia Marina I Gutiérrez

PURPOSE The purpose is to develop a real-time multiplex reverse transcription-PCR assay for detection and quantification of leukemia-specific chimeric transcripts that identify the genetic subgroups of acute lymphoblastic leukemias (ALLs) proposed by the WHO classification. EXPERIMENTAL DESIGN Real-time multiplex assay for t(12;21), t(4;11), and t(1;19) with hypoxanthine phosphoribosyltransfe...

2008
Phaisan Khawsak Sirichai Phantana Kosum Chansiri

A reverse transcriptase-polymerase chain reaction (RT-PCR) and a single-tube multiplex PCR assay was modified for typing of dengue virus in different geographical areas of Thailand during 2000-2001. A set of primers (D1 and D2) was used to generate the RTPCR product of 511 bp in size which subsequently underwent a single-tube multiplex PCR amplification using the highly specific primers for eac...

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