Using DNA sequence data from pathogens to infer transmission networks has traditionally been done in the context of epidemics and outbreaks. Sequence data could analogously be applied to cases of ubiquitous commensal bacteria; however, instead of inferring chains of transmission to track the spread of a pathogen, sequence data for bacteria circulating in an endemic equilibrium could be used to ...

New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla(NDM-1) in China. The isolate is highly resistant to all tested antimicrobials except polymyxin. bla(NDM-1), bla(CTX-...

occurring in South Korea because high similarities (98.6%–99.8%) are found among 4 subspecies of R. conorii. Multilocus sequence typing can help differentiate among these subspecies (6). This study provided a model to amplify SFG rickettsial DNA from sera of patients, and it will be helpful in surveillance of these diseases. However, the results should be interpreted more carefully in the conte...

A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventiona...

Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across the whole genome of C. felis strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA in 17 C. felis isolates, including the C. felis Baker vaccine strain, and 122 clinical samples from different geogr...

Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78...

In this study, a new Vairimorpha isolate was defined from Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) in Turkey (Malatya). Infection determined the host's hindgut, both larval and adult stages. Two types spore originated polymorphic sporulation detected during observations. While binucleate ovocylindrical fresh spores were measured 4.38 ± 0.31 μm (5.09–3.77 μm, n = 100) length 2.80 0.18...

INTRODUCTION Streptococcus pneumoniae is a major cause of mortality and morbidity in young children and the elderly. In the present study we evaluated antimicrobial susceptibilities, serotypes, and sequence types of pneumococcal isolates recovered in New Delhi, India. METHODOLOGY A total of 126 clinical isolates of Streptococcus pneumoniae were investigated. They were subjected to disk diffus...