Caveolin-1 is normally localized in plasma membrane caveolae and the Golgi apparatus in mammalian cells. We found three treatments that redirected the protein to lipid storage droplets, identified by staining with the lipophilic dye Nile red and the marker protein ADRP. Caveolin-1 was targeted to the droplets when linked to the ER-retrieval sequence, KKSL, generating Cav-KKSL. Cav-DeltaN2, an i...

We report an investigation of ordering transitions that are induced in water-dispersed, micrometer-sized droplets of a thermotropic liquid crystal (LC) by the bacterial lipopolysaccharide endotoxin. We reveal that the ordering transitions induced by endotoxin - from a bipolar state of the droplets to a radial state - are strongly dependent on the size of the LC droplets. Specifically, as the di...

The radar Doppler spectrum contains a wealth of information on cloud microphysical properties. Typically, radar-based cloud retrievals use only the zeroth or first moments of the Doppler spectrum – reflectivity and mean Doppler velocity – to derive quantities such as cloud water content and particle characteristic size (e.g., Liou and Sassen 1994; Matrosov et al. 2002). When using only the mome...

OBJECTIVE Adipocyte differentiation-related protein (ADRP)-containing lipid droplets have an essential role in the development of insulin resistance and atherosclerosis. Such droplets form in a cell-free system with a diameter of 0.1 to 0.4 microm, while the droplets present in cells vary in size, from small to very large, suggesting that the droplets can increase in size after being assembled....

This article illustrates the spreading and receding characteristics of non-Newtonian droplets impinging on solid surfaces at different Weber numbers. A xanthan gum solution was used to generate non-Newtonian droplets. From digital images captured using a high speed camera, spreading diameters and dynamic contact angles (DCA) were measured during the impact process. Depending on impact velocity,...

Microfluidics (MFs) offers a promising method for the preparation of polymer microgels with exquisite control over their dimensions, shapes and morphologies. A challenging task in this process is the generation of droplets (precursors for microgels) from highly viscous polymer solutions. Spatial separation of MF emulsification and gelation of the precursor droplets on chip can address this chal...

The surface composition and surface tension of aqueous droplets can influence key aerosol characteristics and processes including the critical supersaturation required for activation to form cloud droplets in the atmosphere. Despite its fundamental importance, surface tension measurements on droplets represent a considerable challenge owing to their small volumes. In this work, we utilize holog...

COVID-19 pandemic promoted a lot of research activities in relation to mucosalivary fluid airborne transport. Indeed, infection mechanisms are the result droplets exchange and knowledge this area is still largely inadequate. One main challenges concerns modelling complex nature. Specifically, key element predict small diameters dry nuclei formation which highly relevant from transmission risk p...

many isotropic oils, this liquid crystal is not soluble in water. We therefore We have prepared monodisperse suspensions of nematic liquid use an ionic surfactant, sodium dodecyl sulfate (SDS), to stabilize the crystal droplets in water. The droplets are stabilized by polyvinyl droplets. This allows us to use standard fractionated crystallization methods alcohol, a polymer that induces planar a...

Light microscopy of living and extracted adrenal cells (Y-1 mouse adrenal tumour cells and cultured bovine fasciculata cells), using Nomarski optics and fluorescence with nile red to stain lipid, revealed in both cell types that lipid droplets remain attached to intermediate filaments when the cells are extracted to prepare these structures. Electron microscopy of thin sections shows the presen...