Nodal segments from six years old plants of Bauhinia variegata L. were cultured on Murashige and Skoogs (MS) medium supplemented with different concentration and combinations of BAP and IBA. Explants culture in MS basal medium supplemented with 5mg/1 BAP showed highest rate of shoot multiplication. When in vitro shoots were inoculated on the MS basal medium supplemented with IBA 2mg/l, profusse...

saffron (crocus sativus l.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of cellulase, pectinase and hemicellulase and embedded in ca-alginate beads. they were cultured with or without nurse cells in ms medium supplemented with 2,4-d and 6-benzylaminopurine at 25°c. after several changes of medium, cell-clusters appeared on the surface of the ca-algi...

Apple is one of the most important temperate-zone fruit trees in the world. Micropropagation of apple scion and rootstocks is an important method of rapid production of healthy and disease-free plants. In this research, in vitro propagation of three apple roottocks including Azayesh-Esfahan, Morabbaee-Mashhad and M9 was investigated. Shoot proliferation carried out in two basal media (MS and WP...

Eclipta alba is a medicinal herb, also known as Bhringraj belonging to family Asteraceae. The plant has hepatoprotective, antimicrobial and hair growth promoting properties. The effect of various phytohormones on in-vitro callus induction and shoot regeneration in Eclipta alba from leaf explant was evaluated. The sterilized explants were inoculated on MS medium supplemented with different conce...

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obta...

Shoot tips and nodal segment explants to Rauwolfia serpentina when cultured on MS medium containing varying concentration of Benzyl adenine purine (BAP) and Benzyl adenine purine in combination with Indole butyric acid (IBA) produced multiple shoots. Maximum multiple shoots (85.6%) were found in static MS medium supplemented with 5.0 mg/ 1 BAP and 0.5 mg / 1 IBA along with 2.5% sucrose and 0.85...

Origanum acutidens (HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0...

A rapid and efficient protocol was developed for inducing direct organogenesis using shoot tip explants of Vernonia cinerea L. Shoot tip explants were cultured on MS medium supplemented with different concentrations of BAP (2.22 to 22.20 μM/l) and KN (2.42 to 23.20 μM/l) for direct shoot induction. The frequency of multiple shoot induction and proliferation increased with increasing concentrati...

Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish n...