inducible nitric oxide synthase (inos) gene expresses a calcium calmudolin-independent enzyme which can catalyse no production from l-arginine. the induction of inos activity has been demonstrated in a wide variety of cell types under stimulation with cytokines and lipopoly saccharide (lps). previous studies indicated that all nitric oxide synthases (nos) activated in human umbilical vein endot...

Abstract Background CVD is the leading cause of death in T2DM patients. However, few biomarkers have been identified to detect and diagnose early stage T2DM. The aim our study was identify important mRNAs, micro (mi)RNAs SNPs (single nucleotide polymorphisms) that are associated with metabolic cardiovascular disease. Materials methods Expression profiles GWAS data were obtained from Gene Omnibu...

ubiquitin - proteasome system (ups), the major protein degradation pathway in the cells, typically degrades short - lived and damaged proteins and regulates growth and stress responses. this pathway is altered in various cancers, including acute lymphoblastic leukemia (all). all begins with a change in bone marrow cells and is the most common type of leukemia in children under 15 years. ube2q1 ...

Introduction: Calcium/calmodulin-dependent protein kinase II (CaMKII) is highly expressed in the hippocampus, which has a pivotal role in reward-related memories and morphine dependence. Methods: In the present study, morphine tolerance was induced in male Wistar rats by 7 days repeated morphine injections once daily, and then gene expression profile of α-isoform of CaMKII (CaMKIIα) in the hipp...

introduction: cryptorchidism has been proved to cause apoptosis in germ cells in respond to changes in the stimulation levels of specific physiological events. the purpose of this study was to determine whether treatment of bilateral cryptorchidism was associated with alterations in testicular gene expression. methods: to induce bilateral cryptorchid model, immature mice were anesthetized and a...

background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...

Pancreatic adenocarcinoma (PAAD) is one of the leading causes cancer-related deaths worldwide. Through data mining, an increasing number biomarkers have been identified to predict survival patients with PAAD. However, ability single gene patient still insufficient. This study aimed develop a novel risk signature for predicting mRNA expression profiling was performed large PAAD cohort (n = 177) ...

Colorectal cancer remains one of the major cancer- related deaths despite progress in the treatment during past decades. Detection of disease at earlier stages reduces its mortality. The aim of current study was to investigate expression of Cytokeratin 19 (CK19), Cytokeratin 20 (CK20) and Guanylyl Cyclase C (GCC) mRNA in peripheral blood of non- metastatic colorectal cancer patients which may r...

sphingosine-1-phosphate (s1p) is a bioactive platelet-derived sphingolipid involved in regulation, proliferation, differentiation, hypertrophy and anti-apoptosis of cells and activation of satellite cells. the aim of the present study was to investigate s1p as a growth factor in response to resistance training in male wistar rats. 24 eight-week-old male wistar rats (190–250 gr.) were used in th...