Thiolutin is commonly used as a general inhibitor of transcription in yeast. It has been used to calculate mRNA decay rates by stopping the transcription and then determining the relative abundance of individual mRNAs at different times after inhibition. We report here that thiolutin is also an inhibitor of mRNA degradation, and thus its use can lead to miscalculations of mRNA half-lives. The i...

Nobody's perfect, and even the cell turns out a certain fraction of erroneous mRNA transcripts. One of the key quality control mechanisms put in place to recognize and eliminate these transcripts before they can be translated into faulty proteins is nonsense-mediated decay. Proteins involved in nonsense-mediated decay are highly conserved across species from plants to humans, and recent studies...

inverse PCR primers, P1 and P2, can be used to delete the same internal region from a series of DNA constructs containing the target region. Similarly, an internal deletion can be made by a PCR-ligation-PCR approach as described by Ali and Steinkasserer (1). According to this method, for example, the first-round PCR can be performed in two seperate reactions by primers V1/P2 and P1/V2, followed...

objective(s): human and animal studies have shown a close relationship between obesity and asthma severity. here, we examined the effects of diet-induced obesity (dio) on the expression levels of il-1β, irak-1 and traf-6 mrna in male wistar rats tracheal after sensitization with ovalbumin (ova). materials and methods:twenty male wistar rats divided to four groups, included, control group with n...

Introduction: In patients with Multiple Sclerosis (pwMS) disease- modifying therapies (DMTs) are known to affect immune response antigens and possibly SARS-CoV2 vaccine. Therefore, post-vaccination serological assessments needed evaluate the effect of vaccine on SARS-CoV-2 antibody response. Objectives aims: We designed a prospective multicenter cohort study enrolling pwMS who were scheduled fo...

Therapeutics based on messenger RNA (mRNA) have become a promising class of methods for various diseases’ treatments in recent years. The safe and effective delivery mRNA into target cells is the premise clinical translation mRNA. Due to protonation ionizable lipids acidic conditions, lipid nanoparticles (iLNPs) lower toxicity higher endosomal escape efficiency than permanent cationic (cLNPs). ...