Acyl CoA diacylglycerol acyltransferase (DGAT, EC 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. It catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl CoA as substrates. The protein sequence of diacylglycerol acyltransferse (DGAT) Type 2B in Moretierella ramanniana var. angulispora (Protein_ID = AAK84180...

BACKGROUND Although some microorganisms can convert glycerol into valuable products such as polyunsaturated fatty acids, the yields are relative low due primarily to an inefficient assimilation of glycerol. Mortierella alpina is an oleaginous fungus which preferentially uses glucose over glycerol as the carbon source for fatty acid synthesis. RESULTS In the present study, we metabolically eng...

Polyurethane foam supports immobilization of Mortierella isabellina ATCC 38063, a zygomycete that hydroxylates and thereby detoxifies resin acids found in certain pulp mill effluents. The fungus becomes entrapped as it threads growing mycelia through the open foam matrix. The tenacity of binding and the amount of biomass bound depend on initial spore numbers and nutrient concentration. Optimal ...

Endosulfan is a broad-spectrum cyclodiene insecticide that has been used extensively for over 30 years on a variety of crops. It is a mixture of two stereoisomers, a and b endosulfan, in a ratio of 7 : 3. Its toxicity is extremely high against fish and aquatic invertebrates, and it has increasingly been implicated in mammalian gonadal toxicity, genotoxicity and neurotoxicity; however, endosulfa...

The accumulation of sewage sludge is a severe problem in many countries. Its utilization through composting has the potential to become widely applied technology. From this perspective, our study investigated diversity fungi mesophilic and thermophilic habitats when biosolids, cow manure wheat straw. It was conducted using metagenomic approach next-generation Illumina HiSeq2000 sequencing revea...

Ilość szkodliwych ksenobiotyków w ekosystemach nieustannie się zwiększa, ze względu na dużą dostępność i wykorzystanie detergentów, pestycydów, farmaceutyków, rozpuszczalników czy ropy naftowej. Takie substancje chemiczne jak fenol, monocykliczne węglowodory aromatyczne, policykliczne alifatyczne oraz związki fosfonoorganiczne są wyjątkowo trwałe niskich temperaturach zwiększoną gęstość mniejsz...

Golden buckwheat ( Fagopyrum dibotrys , also known as F. acutatum ) is a traditional edible herbal medicinal plant with large number of secondary metabolites and considered to be source therapeutic compounds. Different ecological environments have significant impact on their compound content effects. However, little about the interactions between soil physicochemical properties, rhizosphere, en...

Revegetation is regarded as an effective means to improve the ecological environment in deserts and profoundly influences potential functions of soil fungal community. Therefore, Illumina high-throughput sequencing was performed characterize diversity community composition at two depths (0–10 cm 10–20 cm) with four revegetation durations (natural grassland, half-mature, nearly mature, mature Pi...