Molecular Detection of Resistance Determinants Nathan A. Ledeboer* and Richard L. Hodinka Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin; Dynacare Laboratories, Milwaukee, Wisconsin; Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania; and Clinical Virology Laboratory, Children’s Hospital of Phil...

Cell culture and neutralization have been considered the gold standard for enterovirus detection and identification for more than 50 years, but molecular amplification technologies are rapidly replacing the traditional methods in clinical and public health laboratories. Assays based on reverse transcription–polymerase chain reaction or nucleic acid sequence-based amplification may be used to de...

n engl j med 364;2 nejm.org january 13, 2011 182 stem cells is the standard by which their pluripotent potential is evaluated.1-3 We believe that the intended application of human induced pluripotent stem-cell lines should determine the evaluation method. The issue of pluripotency becomes important for studies of the functional mechanism of reprogramming. However, a reproducible and rapid metho...

The determination of antimicrobial susceptibility of a clinical isolate, especially with increasing resistance, is often crucial for the optimal antimicrobial therapy of infected patients. Nucleic acid-based assays for the detection of resistance may offer advantages over phenotypic assays. Examples are the detection of the methicillin resistance-encoding mecA gene in staphylococci, rifampin re...