conclusions optimal wc values for defining mets were 91.5 cm and 85.5 cm in men and women, respectively. the cut-off values recommended by world organizations are not appropriate for an adult population living in ahvaz city. results the cut-off points of wc, according to a maximum sum of sensitivity plus specificity for detecting at least two components of mets were found to be 91.5 cm and 85.5...

Abstract Purpose Metabolic syndrome (MetS) is characterized by visceral obesity, elevated blood pressure and fasting glucose, increased triglycerides, lower high-density lipoprotein cholesterol. MetS related with intricate gene-environment interactions. FTO RETN variants were linked to the occurrence of MetS, but inconsistent results reported. Therefore, this study was conducted evaluate potent...

background: the objective of this study was to investigate the effect of vitrification and in vitro maturation on the mitochondrial distribution and atp content of oocytes. methods: the oocytes at germinal vesicle (gv) and metaphase ii (mii) stages were recovered from 6-8 week old nmri strain female mice. the oocytes were divided into vitrified and non-vitrified groups. vitrification was done b...

Currently, the use of nanotechnology opens up new opportunities to influence processes anaerobic glycolysis and activity hexose monophosphate reactions in preserved erythrocytes. Components containing donor red blood cells on CPDA-1 preservative were examined. Modified solutions 0.9% NaCl with 5% glucose used as resuspending solutions. The treated magnetite nanoparticles (ICNB brand) by Belouso...

5'-(p-(Fluorosulphonyl)[14C]benzoyl)adenosine (FSBA) was synthesized and used as a probe to study the ATP-binding site of smooth-muscle myosin light-chain kinase (MLCK). FSBA modified both free MLCK and calmodulin/MLCK complex, resulting in inactivation of the kinase activity. Nearly complete protection of the calmodulin/MLCK complex against FSBA modification was obtained by addition of excess ...

We develop a riboadenosine-substituted DNA probe-based bioluminescence method for real-time monitoring of exonuclease III (Exo III) activity and Exo III-assisted target recycling. This method employs the target-dependent ATP as the bioluminescence reporter without the involvement of any sophisticated luciferase manipulations, and the glow-type reaction kinetics facilitates the performance of im...

Adenosine triphosphate (ATP) analogues labelled with two dyes suitable for undergoing Förster Resonance Energy Transfer (FRET) have the potential to be valuable tools to continuously study the enzymatic activity of ATP consuming enzymes. Here, we present a synthesis strategy that allows obtaining these ATP analogues in a straight-forward manner. Earlier studies indicate that modifying ATP at th...

Many important cellular membrane fission reactions are driven by ESCRT pathways, which culminate in disassembly of ESCRT-III polymers by the AAA ATPase Vps4. We report a 4.3 Å resolution cryo-EM structure of the active Vps4 hexamer with its cofactor Vta1, ADP·BeFx, and an ESCRT-III substrate peptide. Four Vps4 subunits form a helix whose interfaces are consistent with ATP binding, is stabilized...