the complexation reaction between hydroxyl ammonium (honh3+ ) cation with 18-crown-6 (18c6) ligand was studied in dimethylsulfoxide – water (dmso-h2o), methanol – water (meoh-h2o) and dimethylformamid – methanol (dmf-meoh), binary solutions at different temperatures using conductometric method. the obtained results show that the stoichiometry of the complex formed between honh3+ cation with (18...

Naturally occurring suppressor cells of the in vitro mixed leukocyte culture reaction and of in vivo graft-vs.-host disease have been identified in the spleens of neonatal mice (1) and of adult mice recovering from total lymphoid irradiation (2), whole-body irradiation (3), and syngeneic marrow transplantation (4), or cyclophosphamide therapy (5). Using both positive and negative selection proc...

Dendritic cells (DCs) are highly specialized antigen-presenting cells that play a key role in the initiation and regulation of immune responses. The ability of DCs to process antigens and the outcome of their interaction with T cells are largely dependent on phenotype as well as maturation state of DCs. In this study, we generated DCs from rat bone marrow precursors. Bone marrow cells cultured ...

The utilization of peripheral leukocytes, mixed preparations or specific populations for the detection of homozygous and heterozygous states of inborn errors of metabolism via enzyme assay or detection of accumulated metabolites are reviewed. Additionally, present knowledge of metabolic activities in leukocyte populations and areas of potentially fruitful investigation are discussed.

By CHEV KIDS0N C ATALASE is an enzyme found in most body tissues which has the capacity to release molecular oxygen from hydrogen peroxide ( H202 ) . It is concerned with peroxidation of substrates such as alcohol, phenol, formate and formaldehyde.1 Catalase in blood has aroused considerable interest, the enzyme content being higher than that in other tissues except liver.2 Most studies have co...

Engagement of the CD3/T cell antigen receptor complex on small, resting T cells is insufficient to trigger cell-mediated cytotoxicity or to induce a proliferative response. In the present study, we have used genetic transfection to demonstrate that interaction of the B7-BB1 B cell activation antigen with the CD28 T cell differentiation antigen costimulates cell-mediated cytotoxicity and prolife...

Based on clinical observations, we have previously shown in a murine model that recipient leukocyte infusion (RLI) induces a host-versus-graft reaction in mixed bone marrow chimeras and that rejection of donor cells leads to a specific antitumor response against recipient malignancies. This response is dependent on T cells and IFN-gamma. We investigated the role of NKT cells (NKTs) in this phen...

We studied the effects of hyperthermia (up to 43°)on the cytolytic activity (''Cr release assay) and differentia tion of primary and secondary cytolytic T-lymphocytes (CTL) in vitro. Mixed-leukocyte cultures were established from splenic leukocytes of C57BL/6 mice and DBA/2 stimulator lymphocytes, and the CTL formed on Day 4 (primary) or on Day 21, 3 days after restimulation of the cultures wi...

The TS/A mouse mammary adenocarcinoma is a poorly immunogenic tumor widely used in preclinical models of cancer immunotherapy. CTLs have often been indicated as important in TS/A tumor destruction, but their generation in this model has been rarely studied, nor have their precise target(s) been identified. We hypothesized that the gp70 Env product of an endogenous murine leukemia virus could be...