Growing hairy roots in bioreactors has proven challenging. Here we summarize the recent work using a novel bioreactor, the mist reactor, for the culture of hairy roots from many plant species. Compared to most liquid-phase bioreactors, this gasphase bioreactor offers a completely different environment for growing roots. Design, modeling, and some of the unique engineering aspects inherent to mi...

Shoot tip cuttings of Quercus phillyraeoides A. Gray ‘Emerald Sentinel’ (‘Emerald Sentinel’ oak), in a transitional growth stage between softwood and semi-hardwood, were collected from containerized plants growing under uniform fertility on June 4, 1998. Cuttings were treated with talc formulations of indolebutyric acid (IBA), ranging from 0 to 8000 ppm (0.8%), and placed under intermittent mis...

This work aimed to evaluate the effect of acid mist on microbial communities developed mortar with same composition from wall National Museum in Rio de Janeiro. Five autochthonous groups were inoculated coated acrylic paint and uncoated surfaces submitted or water for 100 days. A heterogeneous biofilm was well coupons under mist. Molecular analysis revealed that bacterial structured according i...

The maximum internal spanning tree (MIST) problem is utilized to determine a in graph G, with the number of possible vertices. incremental (IMIST) version MIST whose feasible solutions are edge-sequences e1, e2, …, en−1 such that first k edges form trees for all ∈ [n − 1]. A solution’s quality measured using $${\text{max}_{k \in - 1]}}\frac{{\text{opt}(G,k)}}{{\left| {\text{In}({T_k})} \right|}...