A large quantity of high quality RNA is often required in the analysis of gene expression. However, RNA extraction from samples taken from woody plants is generally complex, and represents the main limitation to study gene expression, particularly in refractory species like conifers. Standard RNA extraction protocols are available but they are highly time consuming, and not adapted to large sca...

AIM The case of a lower molar with apical periodontitis, which had previous root canal treatment and a fractured instrument in the distal root beyond the foramen, is presented. BACKGROUND The simultaneous presence of a foreign body (endodontic instrument or material) in periapical tissues and microorganisms in the root canal, are etiological factors in the formation or maintenance of a periap...

Nakao, Miki, Kyoichi Ono, Susumu Fujisawa, and Toshihiko Iijima. Mechanical stress-induced Ca21 entry and Cl2 current in cultured human aortic endothelial cells. Am. J. Physiol. 276 (Cell Physiol. 45): C238–C249, 1999.—A fluid stream through a microtube was applied to cultured human aortic endothelial cells to investigate the endothelial responses of both the ionic currents and intracellular Ca...

Electrochemically-propelled nanomotors offer considerable promise for developing new and novel bioanalytical and biosensing strategies based on the direct isolation of target biomolecules or changes in their movement in the presence of target analytes. For example, receptor-functionalized nanomotors offer direct and rapid target isolation from raw biological samples without preparatory and wash...

We developed a portable needle-probe videomicroscope with a charge-coupled device (CCD) camera to visualize the subendocardial microcirculation. In 12 open-chest anesthetized pigs, the sheathed needle probe with a doughnut-shaped balloon and a microtube for flushing away the intervening blood was introduced into the left ventricle through an incision in the left atrial appendage via the mitral ...

The present study investigated the intracanal decontamination and apical extrusion of bacteria debris from root canals instrumented with rotary reciprocating systems (ProDesign Logic or ProDesign R), different file diameters using conventional syringe irrigation (CSI) passive ultrasonic (PUI). Eighty extracted mandibular premolars were contaminated Enterococcus faecalis randomly assigned to eig...

Abstract The keen interest in fuel cells and metal‐air batteries stimulates a great deal of research on the development cost‐efficient high‐performance catalyst as an alternative to traditional Pt boost sluggish oxygen reduction reaction (ORR) at cathode. Herein, we report facile scalable strategy for large‐scale preparation free‐standing flexible porous atomically dispersed Fe–N‐doped carbon m...