UNLABELLED PREMISE OF THE STUDY Nine polymorphic and 12 monomorphic microsatellite loci (simple sequence repeats [SSRs]) were isolated and characterized for the gynodioecious grassland perennial Saxifraga granulata. • METHODS AND RESULTS Based on genomic screening of leaf material of four individuals from four populations, a total of 21 microsatellite primer pairs were designed for S. gran...

The sea cucumber Holothuria leucospilota has high me-dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilot...

UNLABELLED PREMISE OF THE STUDY Twelve microsatellite markers were developed for Primula mistassinica, a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. • METHODS AND RESULTS We used Roche/454 high-throughput technology to sequence micro...

PREMISE OF THE STUDY Microsatellite markers were identified and characterized to study the genetic diversity and structure, conservation status, taxonomy, and biogeography of subspecific taxa and populations of Campanula pyramidalis (Campanulaceae). METHODS AND RESULTS Eleven microsatellite markers were developed from genomic libraries enriched for di- and trinucleotide repeats. A total of 80...

UNLABELLED PREMISE OF THE STUDY Microsatellite loci were developed for Pseudotaxus chienii, an old rare species endemic to China, and which provided a useful tool for investigating the patterns of population genetic structure, phylogeography, evolutionary history, and adaptive potential. Transferability was assayed in the related species, Taxus wallichiana var. mairei. • METHODS AND RESULT...

Manilkara zapota (L.) P. Royen, a widely adapted and popular tree meant for its appetizing fruits in tropics with no genomic resources like microsatellite markers. In order to develop markers primarily Sapota, the study sequenced partial DNA using next generation sequencing technology on Illumina HiSeq 2500 platform. 3.3 Gb data assembled into 6,396,224 contigs were analysed. From these contigs...

Marker-assisted backcross breeding program was anticipated to introgress bacterial blight (BB) resistance genes into an elite fine-grain rice cultivar VGD 1. Pyramided with three (xa5, Xa13, Xa21), ADT 55 the donor parent. A parental polymorphism survey executed between VGD1 and using genome-wide simple sequence repeats (SSR) also called microsatellite markers, identify polymorphic markers. Dis...

The DNA fingerprinting of fruit crops, based on microsatellite markers that are considered to be the key for molecular analysis germplasm collections, is reviewed. Simple sequence repeats (SSRs) remain choice in humans, animals, plants, and other living organisms. This review, considers 44 species, provides a set suitable profiling accessions should make databases produced different laboratorie...

Sixty-six haplotypes at a locus containing a simple dinucleotide (CA)n microsatellite repeat were isolated by PCR-single-strand conformational polymorphism from populations of the horseshoe crab Limulus polyphemus. These haplotypes were sequenced to assess nucleotide variation directly. Thirty-four distinct sequences (alleles) were identified in a region 570 bp long that included the microsatel...

Motivation Microsatellites, also known as short tandem repeats (STRs), are tracts of repetitive DNA sequences containing motifs ranging from two to six bases. Microsatellites are one of the most abundant type of variation in the human genome, after single nucleotide polymorphisms (SNPs) and Indels. Microsatellite analysis has a wide range of applications, including medical genetics, forensics a...