We examined changes in nonstructural carbohydrate biosynthesis and activities of related enzymes in leaves of micropropagated apple plants (Malus domestica Borkh. cv. 'NaganoFuji') in response to water stress, with particular emphasis on the enzymes associated with sorbitol, sucrose and starch metabolism. Water stress resulted in the accumulation of photosynthates in leaves, mainly sorbitol, su...

Walnuts are considered recalcitrant to tissue culture, with a great genetic determinism on all stages of micropropagation; while other factors, also impact, become more complicated the reproduction newly realized varieties. In this research, holistic approach aimed determine influence genotype and nutritive formulation throughout whole cycle micropropagation four Persian walnut varieties (Jugla...

Ectomycorrhiza, a symbiosis between soil fungi and the rootlets of major forest trees, is characterized by well defined anatomical traits but also encompasses a wide range of ecological and physiological situations. Functional studies of this symbiosis therefore address different kinds of systems. Here we review works done on an experimental model with micropropagated oak cuttings infected in a...

Successful in vitro propagation through ex vitro rooting mechanism has been achieved in Piper longum by nodal shoot segment cultures. Shoot tip explants were less proliferative compared to the nodal meristems. The explants were sterilized using 0.1% HgCl2 and cultured on Murashige and Skoog (MS) medium with various concentrations of growth regulators. MS medium supplemented with 1.0 mg/L 6-benz...

Ashwagandha (Withania somnifera) is one of the recognized plant species that considered most traditional natural supplements. Tissue culture an efficient method as fast and affordable in propagation. Few studies have discussed genetic impact such on ashwagandha plant. The aim this research was to identify stability micropropagated plantlets assess vitro-propagation somaclonal variability using ...

Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyrosequencing (TEFAP) analysis amplified sequences from callus homologous to diverse fungal and bacterial...