objective: sea cucumber is a traditional food and medical item and has been reported to exhibit antioxidant, antifungal, anti tumoral and antibacterial bioactivity. the objective of this study is to describe the antibacterial activity of 4 extracts of holoturia. sp (sea cucumber), collected from hengam island of persian gulf. materials and methods: methanol, hexane, aqueous and chloroform extr...

manganese oxide nanoparticles (mn3o4-nps) were prepared using precipitation method. the prepared nanoparticles were characterized by a number of techniques, including x-ray diffraction (xrd) analysis, scanning electron microscopy (sem), transmission electron microscopy (tem) and fourier transforms infra red (ft-ir) spectroscopy. the xrd pattern showed that the structure of mn3o4-nps was tetrago...

A new rapid microdilution method, employing the dye resazurin as an indicator of mycobacterial growth, was developed to evaluate drug susceptibility of Mycobacterium tuberculosis reference strain H37Rv and of 13 M. tuberculosis susceptible or multidrug-resistant clinical strains. Different growth conditions were evaluated. The MICs of isoniazid, rifampicin, streptomycin and ethambutol were dete...

The resistance of Escherichia coli to cephalothin was found to be overestimated when the Phoenix automated susceptibility system was used to determine resistance compared to reference broth microdilution, a finding that jeopardized the use of cephalexin for first-line treatment of urinary tract infections in children. In addition, using broth microdilution, we studied the accuracy of either cep...

Interpretive zone size breakpoints for diffusion tests with 30-micrograms cefotiam disks are diameters of greater than or equal to 18 mm for susceptible and less than or equal to 14 mm for resistant strains. The standard control strains Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 should both give zones 27 to 33 mm in diameter. Tests with 579 clinical isolates yielded an una...

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution r...

We modified the phosphocellulose binding assay for aminoglycoside-modifying enzymes (AMEs) by use of microdilution plates and a multichannel micropipette. Batteries of aminoglycoside substrates for screening organisms for the presence of AMEs as well as for subclassifying enzymes were prepared and stored in microdilution plates. When tested in parallel with the conventional tube reaction assay,...

Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization, hybridized DNAs were quantitatively detected with peroxidase-conjugated stre...

International Journal of Medicine and Public Health,2022,12,4,175-179.DOI:10.5530/ijmedph.2022.4.32Published:January 2023Type:Original ArticleDetection Colistin Resistance in Gram Negative Pathogens: A One Year Cross-sectional Study a Tertiary Care Centre Northeast IndiaDeepayan Biswas, Wihiwot Valarie Lyngdoh, Sheryl Lanong, Clarissa Jane Prithwis Bhattacharyya, Nari Mary Lyngdoh Deepayan Bisw...

There are at least four different existing or proposed interpretive criteria for the disk diffusion susceptibility testing of ticarcillin and ticarcillin plus clavulanic acid (T/C). To assess these criteria, 570 gram-negative bacillary isolates were tested for susceptibility to ticarcillin and T/C by both disk diffusion and broth microdilution methods. These included 53 strains of the family En...