Chymosin is a predominant enzyme in rennet and used cheese production because of its excellent milk-clotting activity. Herein, we proposed facile label-free electrochemical method for determining chymosin activity based on peptide-based substrate. The synthesized substrate peptide was assembled onto the surface Au-deposited grassy carbon electrode. current proportional to activity, thus could b...

THE FOLLOWING GENERAL CONCLUSIONS MAY BE DRAWN FROM THE PRECEDING WORK: Fibrin is altered by formaldehyde and is then less easily digested by pepsin and by trypsin. Papaïn is apparently unable to digest fibrin even when this is exposed to very weak formaldehyde (1:1000) for a very short time. The casein of milk, on contact with formaldehyde, undergoes rapid alteration and is as a result not coa...