1. Two enzymes (A and B) which catalyse the sulphation of p-nitrophenol and l-tyrosine methyl ester have been isolated from female rat livers. One of these enzymes (A) also catalyses the sulphation of dehydroepiandrosterone. 2. The K(m) values for the sulphation of p-nitrophenol and l-tyrosine methyl ester by enzyme B at pH7.5 are 1.5mum and 2.9mm respectively. 3. Enzyme B is oxidized on keepin...

Nitric oxide stimulates intestinal ion transport via the activation of enteric nerves, but it is not known whether it regulates intestinal transport function by acting on the epithelium directly. The aim of this study was to determine the influence of nitric oxide on epithelial electrogenic ion secretion, measured as the short-circuit current (Isc), using the human colonic carcinoma cell line C...

A new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichi...

Two purified isozymes of protein carboxyl methyltransferase from bovine brain catalyze the substoichiometric transfer of methyl groups in vitro from S-adenosyl-L-[methyl-3H]methionine to several erythrocyte membrane proteins, which include bands 2.1, 3, and 4.1, as well as several integral membrane polypeptides. D-Aspartic acid beta-[3H]methyl ester has been isolated from proteolytic digests of...

A synthesis of the tetrapeptide sequence As-Ag of glucagon is described that employs various blocking groups, coupling procedures, and routes. The threonyl-phenylalanyl-threonyl-seryl sequence A5-A8 in the hyperglycemic hormone, glucagon (Foa and Galansino, 1962), has been prepared by three different groups within the past four years (Beyerman and Bontekoe, 1964; Schroder and Gibian, 1962; Wuns...

As part of an ongoing investigation into the development of N-substituted amino acids as building blocks for dynamic combinatorial chemistry, we report the structure of the title compound, C(19)H(19)NO(3). This compound crystallizes as discrete mol-ecules. The cinnamoyl group is non-planar, with the phenyl ring and the amide twisted out of the ethyl-ene plane. The benzyl and ester groups lie ab...

A synthesis of the tetrapeptide sequence As-Ag of glucagon is described that employs various blocking groups, coupling procedures, and routes. The threonyl-phenylalanyl-threonyl-seryl sequence A5-A8 in the hyperglycemic hormone, glucagon (Foa and Galansino, 1962), has been prepared by three different groups within the past four years (Beyerman and Bontekoe, 1964; Schroder and Gibian, 1962; Wuns...

Biodiesel fuels consist of a mixture different fatty acid esters. The thermophysical properties the esters are decisive for combustion and storage. Especially density viscosity influence, e.g., energy density, spray quality lubrication in diesel engine. For some esters, several studies on can be found literature wide pressure reasonably temperature range. However, data at high pressures as well...