A fluorescent labeling technique has been devised to quantitate dihydrofolate reductase levels in individual cells and has been used to examine the heterogeneity in dihydrofolate reductase levels within various populations of methotrexate-resistant and parental cells. Precise quantitation of cellular dihydrofolate reductase levels in parental and methotrexate-resistant cells (having up to sever...

background: methotrexate (mtx) is an anti-metabolite drug widely used in treatment of neoplastic disorders, rheumatoid arthritis and psoriasis. the ester derivative, ethyl pyruvate (ep) is stable in solution and should function as an antioxidant and energy precursor. this study was conducted to evaluate the protective role of ep on sperm parameters, testosterone level and malondialdehyde (mda) ...

The potential value ofthe antifolate methotrexate (N1O-methylaminopterin) in the treatment of rheumatoid arthritis (RA) was first recognised in 1951.' The low dose drug administration in divided doses 12 hours apart was developed for the treatment of patients with psoriasis, based on properties of psoriatic epithelial cells and the biological action of methotrexate on these cells.2 To date its ...

OBJECTIVE The objective of this study was to compare the effectiveness of a combination of tumour necrosis factor alpha (TNFalpha) inhibitors with either methotrexate or leflunomide in the treatment of patients with rheumatoid arthritis in a real-world setting. METHODS Data from 1769 outpatients enrolled in the German biologics register RABBIT who were treated with one of the TNFalpha inhibit...

Activity and fluorescence studies have been performed on dihydrofolate reductase from a methotrexate-resistant strain of Escherichia coli B. Whereas the activity of this enzyme does not depend on the sequence of addition of substrate and cofactor, inhibition by methotrexate has been found to be dependent on preincubation conditions, as well as on ionic strength and dihydrofolate concentration. ...

We recently cloned and characterized the rat kidney-specific organic anion transporter, OAT-K1, which was suggested to mediate renal tubular transport of methotrexate. In this study, we investigated the interactions of nonsteroidal anti-inflammatory drugs (NSAIDs) with OAT-K1 by evaluating the effects of these drugs on renal distribution of methotrexate in vivo, and on methotrexate accumulation...

Six-day-old rats were treated intraperitoneal injections with methotrexate 1 mg/kg, and the cerebellum was examined. Both the length and width of the vermis decreased in the methotrexate-treated group instead of the control from 4 day after treatment (DAT) onward. A significant reduction in the width of the external granular layer was detected on 2 and 3 DAT in the methotrexate group. By 4 DAT,...

Methotrexate is a widely used cytostatic in chemotherapy cocktails for the treatment of cancer but is associated with cognitive impairment. Previous animal studies indicated that methorexate decreases hippocampal cell proliferation, which might contribute to the observed cognitive impairment. However, clinical studies have shown that cognitive impairment can also be noticed in some cancer patie...

Methotrexate continues to be one of the most widely used systemic immunosuppressive agents in dermatology. In addition to the important, well-characterized adverse effects such as hepatotoxicity and myelosuppression, methotrexate may induce a number of rare cutaneous adverse events including methotrexate-induced ulceration. We present a case of methotrexate-induced cutaneous ulceration in a pat...

BACKGROUND Efficacy of topical methotrexate in psoriasis is limited by its penetration. AIMS The study involved the preparation of niosomal methotrexate in chitosan gel and to test the same for irritation and sensitization on healthy human volunteers followed by assessing the efficacy of the gel through double-blind placebo-controlled study on psoriasis patients and also comparing its efficac...