Objective Determining the prevalence of nasal carriage of S. aureus, both sensitive to methicillin and resistant to it, in preschool children and evaluating the presence of Panton-Valentine leukocidin genes in the isolates. Methods This was a cross-sectional study in which cultures from anterior nares were obtained from healthy preschool children. Isolates were identified as S. aureus based on ...

It was shown earlier that Tn551 inserted into the C-terminal region of murE of parental methicillin-resistant Staphylococcus aureus strain COL causes a drastic reduction in methicillin resistance, accompanied by accumulation of UDP-MurNAc dipeptide in the cell wall precursor pool and incorporation of these abnormal muropeptides into the peptidoglycan of the mutant. Methicillin resistance was re...

One hundred and eleven isolates of methicillin-resistant Staphylococcus aureus recovered from patients at the Olin E. Teague Veterans Center from March 1983 to April 1987 were as susceptible to lysis by lysostaphin as methicillin-susceptible S. aureus controls were.

methicillin-resistant staphylococcus aureus (mrsa) is the main cause of hospital infection emerged over the last decades. rapid detection of mrsa is important for patient care and proper usage of infection control. detection of meca genes (encoding resistance to methicillin and other similar antibiotics) and nuc genes (encoding staphylococcal thermostable nuclease) by pcr method is now consider...

conclusions there were diverse phenotypic changes among vancomycin-non-susceptible s. aureus isolates. this may be due to the variety of related regulatory systems. the diversity of phenotypic expression may result in its misidentification in routine laboratory checks. materials and methods a total of 130 methicillin-resistant s. aureus (mrsa) isolates were studied, including 49 vssa, 28 hvisa,...

conclusions the present study showed a high prevalence of aminoglycoside-resistance genes among mrsa isolates in two cities in iran. results the results of drug susceptibility testing showed that the highest rate of resistance was against erythromycin in tehran (84.4%) and gentamicin (71.7%) in sari. all isolates were sensitive to vancomycin, and all strains harbored the meca gene. the aac (6’)...

background: methicillin resistant staphylococcus aureus (mrsa) is one of the major agents for increasing number of serious hospital and community acquired infections. the aim of this study was to investigate the occurrence of the mrsa and meca gene among nosocomial and environmental specimens in kurdistan hospitals and determining the antibiotic resistance of the isolates. materials and methods...

background : infections resulted from multi-resistant staphylococcus aureus are increasing worldwide. in the present study, a multiplex-pcr assay for the detection of antibiotic resistance genes among s. aureus clinical isolates and for the concomitant identification of these isolates was described. methods : a total of 127 s. aureus isolates were collected from clinical specimens at three teac...

background and aims: the aim of present study was to investigate the molecular characteristic of staphylococcus aureus to detect mec a gene and to type sccmec in strain isolated from healthy worker at larestan hospital. material and methods: this study was carried out from the 250 nasal swab healthy worker  at larestan hospital. multiplex polymerase chain reaction for meca gene was performed in...

Resistance to methicillin was transduced by phage 80 or 53 from two naturally occurring methicillin-resistant strains of Staphylococcus aureus to methicillin-susceptible recipient strains at frequencies of 10(-7) to 10(-9). Ultraviolet irradiation of transducing phage and posttransductional incubation at 30 C were essential for useful frequencies of transduction. Effectiveness as a recipient fo...