This study aimed to investigate the effect of bioaugmentation on microbial community and function in a continuous anaerobic process treating lignocellulosic cow manure. One reactor (Rb) received dosage for certain period (d100-d170) stopped afterward (d170-d220), while same applied control (Rc) except sterilized was introduced. Samples were taken day130, 170 220 from both reactors metatranscrip...

Extremely low concentrations of chloroform and carbon tetrachloride and somewhat larger concentrations of methylene chloride inhibited the formation of methane by the rumen microbiota in the presence or absence of added substrate. The accumulation of hydrogen at these low concentrations indicates a selective inhibition of methanogenesis. Presumably, these inhibitors affect one or more of the re...

Methane production has been measured from lambs fed contrasting forages. This work has been driven by the need to reduce greenhouse gas emissions from agriculture and to determine energy losses to methane from contrasting diets. Young ram lambs were fed either fresh ryegrass/white clover pasture, lucerne (also pelleted lucerne), sulla, chicory, red clover, Lotus pedunculatus (lotus) and mixture...

Ruminant production is under increased public scrutiny in terms of the importance of cattle and other ruminants as major producers of the greenhouse gas methane. Methanogenesis is performed by methanogenic archaea, a specialised group of microbes present in several anaerobic environments including the rumen. In the rumen, methanogens utilise predominantly H2 and CO2 as substrates to produce met...

A bacterial consortium capable of sucrose degradation primarily to CH(4) and CO(2) was constructed, with acetate as the key methanogenic precursor. In addition, the effect of agar immobilization on the activity of the consortium was determined. The primary fermentative organism, Escherichia coli, produced acetate, formate, H(2), and CO(2) (known substrates for methanogens), as well as ethanol a...

Except when otherwise noted, the free energy changes given for methanogenic reactions were calculated from the free energies of formation from the elements of the substrates and products with non-gaseous compounds at 1 M aqueous solution and gaseous compounds in the gaseous state at 1 atmosphere pressure (101 kPa). The free energy changes of formation were taken from Thauer eta/ . (1977). repor...

Most probable numbers (MPNs) of methanogens in various salt marsh and estuarine sediments were determined with an anaerobic, habitat-simulating culture medium with 80% H(2) plus 20% CO(2) as substrate. Average MPNs for the short Spartina (SS) marsh sediments of Sapelo Island, Ga., were maximal at the 5- to 7-cm depth (1.2 x 10/g of dry sediment). Populations decreased to approximately 880/g of ...

Emissions of methane (CH4), a major greenhouse gas, should be cut by at least 30% 2030 according to the last Conference Parties, CoP26. Aquaculture pond is CH4 emitter, yet microbial mechanisms ruling methanogenesis degradation organic matter in sediments remain unclear. In particular, respective roles hydrogenotrophic and acetoclastic methanogenesis, impact aquaculture farming practices are un...