Kinetochores regulate the dynamics of attached microtubule bundles (kinetochore-fibres, K-fibres) to generate the forces necessary for chromosome movements in mitosis. Current models suggest that poleward-moving kinetochores are attached to depolymerising K-fibres and anti-poleward-moving kinetochores to polymerising K-fibres. How the dynamics of individual microtubules within the K-fibre relat...

Mitosis requires dynamic attachment of chromosomes to spindle microtubules. This interaction is mediated largely by kinetochores. During prometaphase, forces exerted at kinetochores, in combination with polar ejection forces, drive congression of chromosomes to the metaphase plate. A major question has been whether kinetochore-associated microtubule motors play an important role in congression....

Centromere positions on the metaphase plate of 48 root-tip cells of four grass species were analysed using metaphases reconstructed from electron micrographs of serial thin sections. Centromere alignment was almost perfect on a plane in 15 untreated metaphases of cereals where 2n = 14; only 2% of the total variance in centromere position was out of the plane of the metaphase plate. In 23 simila...

The karyological features of Akis subtricostata was determined for the first time with conventional and silver nitrate staining. diploid number 2n=16 meioformula 7+neoXY represents a deviation from modal karyotype Coleoptera. pericentromeric heterochromatin detected both Giemsa In addition to determining single possible NOR on prophase I nuclei, AgNO3 revealed that several telomeric regions mit...

Laser UV microirradiation of Chinese hamster interphase cells combined with caffeine post-treatment produced different patterns of chromosome damage in mitosis following irradiation of a small area of the nucleus that may be classified in three categories: I)intact metaphase figures, II)chromosome damage confined to a small area of the metaphase spread, III)mitotic figures with damage on all ch...