Cadmium (Cd) is an important toxicant in estuarine and coastal environments that can strongly affect energy balance of aquatic organisms by increasing the organism's basal energy demand and reducing its aerobic capacity. Mechanisms of cadmium-induced increase in basal metabolic costs are not well understood and may involve elevated detoxification costs due to the synthesis of cellular protectiv...

1. General introduction 2. General consideration in choosing target genes for phytoremediation 3. Genetic engineering of metal transporters 3.1. Uptake of Metal from the Soil to the Root 3.2. Translocation from the Root to the Shoot 3.3. Vacuolar Transporters 3.4. Manipulating Metal Distribution: Some Aspects 4. Genetic engineering of metal chelation 4.1. Glutathione 4.2. Phytochelatin 4.3. Met...

In rodents, the metallothionein (MT) family is composed of four members, MT-1 to MT-4. MT-1&2 are expressed in virtually all tissues including those of the Central Nervous System (CNS), while MT-3 (also called Growth Inhibitory Factor) and MT-4 are expressed prominently in the brain and in keratinizing epithelia, respectively. For the understanding of the physiological functions of these protei...

Metallothionein is a ubiquitous protein with a wide range of proposed physiological roles, including the transport, storage and detoxification of essential and nonessential trace metals. The amino acid sequence of isoform 2a of rabbit liver metallothionein, the isoform used in our spectroscopic studies, includes 20 cysteinyl groups out of 62 amino acids. Metallothioneins in general represent an...

Lobsters were fed cadmium-rich oysters for 28 days, and the induction of cadmium metallothionein and its relation to concentrations of cadmium, copper, and zinc in the digestive gland and gills was determined. A portion of the tissues also was retained for determining the cytosolic distribution of these metals by gel filtration and ion-exchange chromatography. The digestive gland contained a ma...

Among the heavy metal-binding ligands in plant cells the phytochelatins (PCs) and metallothioneins (MTs) are the best characterized. PCs and MTs are different classes of cysteine-rich, heavy metal-binding protein molecules. PCs are enzymatically synthesized peptides, whereas MTs are gene-encoded polypeptides. Recently, genes encoding the enzyme PC synthase have been identified in plants and oth...