Chloroplast number per cell is a frequently examined quantitative anatomical parameter, often estimated by counting chloroplast profiles in two-dimensional (2D) sections of mesophyll cells. However, a mesophyll cell is a three-dimensional (3D) structure and this has to be taken into account when quantifying its internal structure. We compared 2D and 3D approaches to chloroplast counting from di...

In order to deeply understand the effect mechanism of rapid drought stress on physiological and biochemical properties crop leaves determine thresholds, potted spring wheat under two water treatments, adequate supply continuous stress, was researched. early stage drought, parameters decreased in stomatal conductance (gs), intercellular CO2 concentration (Ci), maximum electron transfer rate (Jma...

Mesophyll conductance ( g m ) generally correlates with photosynthetic capacity, although the causal relationship between the two is unclear. The response of g m to various CO 2 regimes was measured to determine its relationship to environmental changes that affect photosynthesis. The overall effect of CO 2 growth environment on g m was species and experiment dependent. The data did not statist...

The response of mesophyll conductance to CO(2) (g(m)) to environmental variation is a challenging parameter to measure with current methods. The 'variable J' technique, used in the majority of studies of g(m), assumes a one-to-one relationship between photosystem II (PSII) fluorescence and photosynthesis under non-photorespiratory conditions. When calibrating this relationship for Populus trich...

Mesophyll conductance to CO(2) (g(m)) limits carbon assimilation and influences carbon isotope discrimination (Delta) under most environmental conditions. Current work is elucidating the environmental regulation of g(m), but the influence of g(m) on model predictions of Delta remains poorly understood. In this study, field measurements of Delta and g(m) were obtained using a tunable diode laser...

The content of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) (Et; EC 4.1.1.39) measured in different-aged leaves of sunflower (Helianthus annuus) and other plants grown under different light intensities, varied from 2 to 75 &mgr;mol active sites m-2. Mesophyll conductance (&mgr;) was measured under 1.5% O2, as well as postillumination CO2 uptake (assimilatory charge, a gas-exchange ...