A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described fo...

Procedures have been developed for estimating cystine content of proteins by reducing disulfide bonds with mercaptoethanol and selectively alkylating the generated sulfhydryl groups with 4-vinylpyridine or 2-vinylquinoline. 4-Vinylpyridine reacts with cysteine to produce S-,B-(4-pyridylethyl)cysteine (Pe-cysteine), which can be determined in protein hydrolysates either by ion-exchange chromatog...

Erwinia amylovora infected with bacteriophage ERA103 produced an enzyme which degraded the extracellular polysaccharide of noninfected cells. The depolymerase enzyme was purified 15-fold by a procedure which included ammonium sulfate precipitation, ultracentrifugation, CM-Sephadex batchwise separation, Sephadex G-50 column chromatography, and Sephacryl S-200 column chromatography. The enzyme ha...

proteinase K and was then incubated for 4 hrs at 55•Ž . After approximately 30 mg of nail clippings in a 1.5 ml microtest tube were rinsed once with 1.0 N NaOH and twice with distilled water, the nails were mixed with 300ƒÊ1 of 4 M guanidine thiocyanate, 0.5% (w/v) sodium N-lauroyl salcosinate, 25 mM sodium citrate, and 0.1 mM 2-mercaptoethanol [7], and were incubated at room temperature over n...

Methods. Various laboratory tests were implemented in our lab, as screening or confirmatory tests, some in studies some as a routine diagnostic test, such are: Agglutinations tests: Rose Bengal test (RBT), Slide Agglutinations test, Standard tube test (SAT), Wright test, Antihuman globulin test (Coombs); 2-Mercaptoethanol test (2 ME); Complement fixation test (CFT); Indirect enzyme immunoassay ...

The reaction of [(arene)RuCl2]2 (arene /C6H6, p -Me /C6H4 / i Pr, C6Me6) with p -thiocresol, 2-mercaptoethanol and p mercaptophenol in refluxing ethanol gives the tris-thiolato complexes [(arene)2Ru2(SR)3] (R /p -C6H4 /Me, CH2CH2OH, p C6H4OH) which can be isolated as the chloride salts. The remarkable selectivity of (arene)ruthenium units for SH versus OH functions reflects the affinity of ruth...

Immunoglobulin M hemagglutinin and antibody cytophilic for macrophages were produced simultaneously in mice after primary antigenic stimulation with sheep erythrocytes in saline. Antigen emulsified in Freund's complete adjuvant induced formation of cytophilic antibody (CA) in high titer. Purified cytophilic globulin was isolated by permitting it to attach to macrophages in vitro and eluting it ...

Large amounts of Cu-metallothionein were obtained by 2-mercaptoethanol and sodium dodecyl sulphate extractions of the particulate fractions of the liver of pigs given high-Cu2+ diets or rats injected with Cu2+. Three isoproteins were purified from pig liver and characterized on the basis of their physicochemical properties, metal content and amino acid composition. No such pool of Cu-metallothi...

We have measured the linear electric field effect for peroxide-activated yeast cytochrome c peroxidase and for the mercaptoethanol derivative of Neurospora tyrosinase. Although both of these materials have EPR spectra resembling those of free radicals, the linear electric field effect measurements demonstrate that there is a metal ion associated with the paramagnetic centers. In addition, we ha...