نتایج جستجو برای: mammalian expression system

تعداد نتایج: 3050003  

2015
Vincent J. Lynch Mauris C. Nnamani Aurélie Kapusta Kathryn Brayer Silvia L. Plaza Erik C. Mazur Deena Emera Shehzad Z. Sheikh Frank Grützner Stefan Bauersachs Alexander Graf Steven L. Young Jason D. Lieb Francesco J. DeMayo Cédric Feschotte Günter P. Wagner

A major challenge in biology is determining how evolutionarily novel characters originate; however, mechanistic explanations for the origin of new characters are almost completely unknown. The evolution of pregnancy is an excellent system in which to study the origin of novelties because mammals preserve stages in the transition from egg laying to live birth. To determine the molecular bases of...

Journal: :Current Protocols in Protein Science 1997

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2000
T Shioda S Andriole T Yahata K J Isselbacher

An improved mammalian two-hybrid system designed for interaction trap screening is described in this paper. CV-1/EBNA-1 monkey kidney epithelial cells expressing Epstein-Barr virus nuclear antigen 1 (EBNA-1) were stably transfected with a reporter plasmid for GAL4-dependent expression of the green fluorescent protein (GFP). A resulting clone, GB133, expressed GFP strongly when transfected trans...

Journal: :Journal of Visualized Experiments 2012

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