Extracting quantitative measurements from time-lapse images is necessary in external feedback control applications, where segmentation results are used to inform algorithms. We describe ChipSeg, a computational tool that segments bacterial and mammalian cells cultured microfluidic devices imaged by microscopy, which can be also the context of control. The method based on thresholding uses same ...

Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a  chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...

Background Despite successful cloning of many mammalian species, the cloning efficiency is extremely low compared to that of IVF raising the possibility of the existence of epigenetic barrier preventing successful cloning. MaterialsAndMethods Using comparative transcriptome analysis comparing transcriptomes of IVF and SCNT embryos and that of donor cells, we identified epigenetic barrier and fi...

DNA manipulation is crucial for many biotechnological prospects and medical applications such as gene therapy. This requires the amplification extraction of from bacteria transfer these molecules into cells, including bacterial mammalian cells. The capacity natural magnesium silicate clay mineral sepiolite to bind makes it a potentially useful tool biotechnological/medical strategies. In additi...

Introduction Cholinergic system is one of the important systems of mammalian CNS. Cholinergic neurons distributed in brain and spinal cord and contributed to principal functions like: consciousness, learning and memory, and motor control. In this study we investigated the differentiation potentiality of mouse embryonic stem cells toward cholinergic neurons. The aim of this study was to evaluate...

An increasing number of new subtypes avian influenza viruses (AIVs) are reported to be infecting humans, including H3N2, H5N1, H7N9, H10N8, and the recently emerged H3N8 virus in China 2022. However, genetic biological properties currently prevalent AIVs not yet fully understood. This study reports isolation a novel triple reassortment (GD-H3N8) from chicken flocks Guangdong province, China, Th...

calcineurin (can) is a ca2+/calmodulin (ca2+/cam)-dependent serine/threonine protein phosphatase expressed in most mammalian tissues but found at higher concentration in brain. in the last decade there have been a steadily increasing number of studies identifying neuronal can as a primary suspect in neuronal vulnerability, synapse loss, dendritic atrophy, synaptic dysfunction and neuroinflammat...

Activated and promoter-bound heat-shock transcription factor 1 (HSF1) induces RNA polymerase II recruitment upon heat shock, this is facilitated by the core Mediator in Drosophila yeast. Another module, CDK8 kinase module (CKM), consisting of four subunits including MED12 CDK8, plays a negative or positive role regulation transcription; however, its involvement HSF1-mediated remains unclear. We...