anthracnose caused by colletotrichum gloeosporioides s. l. is one of the most important diseases of citrus in northern iran. to study the genetic structure of c. gloeosporioides s. l. from citrusspp., infected samples were collected from three citrus cultivating regions of northern iran, during the summer of 2009. fifty one monoconidial isolates were used as the objective of rep- and box-pcr fi...

Lactobacilli were isolated from jejunal chyme from five fistulated beagles. Cultivable lactobacilli varied from 10(4) to 10(8) CFU/ml. Seventy-four isolates were identified by partial 16S rRNA gene sequencing and differentiated by repetitive element PCR (Rep-PCR), Lactobacillus acidophilus was dominant, and nearly 80% of 54 isolates shared the same DNA fingerprint pattern.

a collection of enterococci  spp. (about 96 isolates) were isolated from two iranian raw milk cheeses, namely lighvan and koozeh, and subsequently, identified as enterococcus faecium , enterococcus faecalis , enterococcus durans , enterococcus casseliflavus and enterococcus italicus by 16s rdna sequencing. all of the 96 isolates were subjected to agar-spot and well-diffusion assay in order to d...

The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ducks, cows, pigs, chickens, and sheep). Our initial studies revealed that the...

Adeno-associated virus type 2 (AAV) is known to establish latency by preferential integration in human chromosome 19q13.42. The AAV non-structural protein Rep appears to target a site called AAVS1 by simultaneously binding to Rep-binding sites (RBS) present on the AAV genome and within AAVS1. In the absence of Rep, as is the case with AAV vectors, chromosomal integration is rare and random. For...

The Lactobacillus plantarum and pentosus genotypes existing in industrial-scale cucumber fermentations were defined using rep-PCR-(GTG)5. ability of each genotype to ferment cucumbers under various conditions was evaluated. Rep-PCR-(GTG)5 the technique capable illustrating most intraspecies discrimination compared sequencing housekeeping genes (recA, dnaK, pheS rpoA), MLST RAPD with primers LP1...