The research included seven autochthonous apple varieties in the area of Tomislavgrad, Stipanjići locality, during 2018, which represent natural wealth our country. In varieties: Petrovača bijela, Budimka, Ljutika-Divljaka, Ledarica, Bedrica, Ljepocvjetka-Cvjetača and Jonatanka, phenological characteristics were monitored analyzed with morphological-pomological properties fruits. following moni...

A major protease present in dormant bark tissues of the apple (Malus domestica Borkh. cv. "Golden Delicious") was partially purified by hemoglobin-coupled Sepharose column chromatography. The protease active at pH 4.6 and at temperatures of 30 to 50 C was found to be sulfhydryl-dependent. Phenylmercuric acetate inhibited the enzyme approximately 50% at 2 millimolar, whereas phenylmethylsulfonyl...

Effects of 1-methylcyclopropene (1-MCP), 2-chloroethyl phosphonic acid (ethephon), and methyl jasmonate (MeJA) on production of aroma volatile compounds and ethylene by ‘Delicious’ and ‘Golden Delicious’ apples [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] during ripening were investigated. Forty-four volatile compounds in ‘Delicious’ and 40 compounds in ‘Golden Delicious’ were d...

Genomic DNA represents the total genome of an organism and is valuable for different molecular studies. The isolation of high quality DNA is a prerequisite for these molecular techniques. The seeds of apple (Malus domestica) contain high amount of polysaccharides, polyphenols and other secondary metabolites that can hamper DNA isolation, amplification, restriction digestion and subsequent molec...

High temperature is one of the most important environmental factors that affect the successful cultivation of apple (Malus × domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation by high temperature treatment was investigated in the fruits of “Fuji” apple at different developmental stages in different temperature conditions through realtime PCR. In ...

Several timbers and crop by-products were subjected to an enzymatic treatment to obtain a xylo-oligosaccharides-enriched preparation. The process was performed by means of the commercial endo-β-1,4xylanase Buzyme 2511. The enzymatic cocktail was applied onto the raw ground materials with yield up to 5.3 ± 1.0 g/L xylo-oligosaccharides for apple pomace. In order to make the materials more access...