Complicaciones a nivel de una porción de la membrana de Bowman durante la rutina del procedimiento LASIK: dos casos que demuestran la ventaja clínica del uso de Mitomicina C en tales casos

TAL effectors (TALEs) contain a modular DNA-binding domain that is composed of tandem repeats. In all naturally occurring TALEs, the end of tandem repeats is invariantly a truncated half repeat. To investigate the potential role of the last half repeat in TALEs, we performed comparative molecular dynamics simulations for the crystal structure of DNA-bound TALE AvrBs3 lacking the last half repea...

Biological computing circuits can enhance our ability to control cellular functions and have potential applications in tissue engineering and medical treatments. Transcriptional activator-like effectors (TALEs) represent attractive components of synthetic gene regulatory circuits, as they can be designed de novo to target a given DNA sequence. We here demonstrate that TALEs can perform Boolean ...

Transcription Activator-Like Effectors (TALEs) of Xanthomonas bacteria are programmable DNA binding proteins with unprecedented target specificity. Comparative studies into TALE repeat structure and function are hindered by the limited sequence variation among TALE repeats. More sequence-diverse TALE-like proteins are known from Ralstonia solanacearum (RipTALs) and Burkholderia rhizoxinica (Bat...

Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate transcription of host genes required for infection success. Here, we show that the basal transcri...

A In Japan, most of children haven’t read the Fairy Tales or tales of old Japan because the high technology video games are more exciting than most of picture books. But they must be effective to bring up the children’s cultivation of aesthetic sensitivity. And we have heard from teachers of elementary schools that most of themes of computer education in school are the operation of Painting Too...

nAture methods | ADVANCE ONLINE PUBLICATION | We tested whether transcription activator–like effectors (tAles) could mediate repression and activation of endogenous enhancers in the Drosophila genome. tAle repressors (tAlers) targeting each of the five even-skipped (eve) stripe enhancers generated repression specifically of the focal stripes. tAle activators (tAleAs) targeting the eve promoter ...

This arts-based study serves two interrelated purposes. First, it draws from multiple tales to illustrate reflexivity (and its benefits) between the author, research topic, and participants. Different forms of tales show multiple and diverse human interconnections and the complexity of understanding potential leisure among sex offenders. The importance of prioritizing reflexivity should not be ...

The ability to perturb individual genes in genome-wide experiments has been instrumental in unraveling cellular and disease properties. Here we introduce, describe the assembly, and demonstrate the use of comprehensive and versatile transcription activator-like effector (TALE) libraries. As a proof of principle, we built an 11-mer library that covers all possible combinations of the nucleotides...