The expression of cytokeratins 18 and 19 by human retinal pigment epithelial cells (HRPE) has been suspected of being associated with HRPE proliferation. We have investigated the involvement of these cytokeratin subtypes in the proliferative and migratory behaviour of cultured HRPE. Cell proliferation markers (bromodeoxyuridine and proliferating cell nuclear antigen) and the cytokeratins were i...

Abstract Background Cytokeratin 18 (CK18) is an intermediate filament protein of the cytokeratin acidic type I group and primarily expressed in single-layered or “simple” epithelial tissues carcinomas different origin. Methods To systematically determine CK18 expression normal cancerous tissues, 11,952 tumor samples from 115 types subtypes (including carcinomas, mesenchymal biphasic tumors) as ...

The objective of this study was to establish a primary culture of omasal epithelial cells (OECs) derived from dairy calves and to characterize its function in small peptide absorption. Bovine omasal tissues were obtained from newborn Chinese Holstein calves and digested with a 2.5% trypsin solution to obtain OECs. The isolated cells were later cultured in DMEM containing 10% fetal bovine serum,...

Cytokeratin positive cells are frequently found in the blood (BL) and bone marrow (BM) of patients with epithelial cancers attributed to metastasis. We document here reproducible presence (ECs) normal human murine BL BM. used four different methods: immunofluorescence microscopy (IF), Krt1-14;mTmG transgenic mice, rtPCR, flow cytometry. To this end, we have made several novel interesting findin...