Twenty-four patients who experienced 26 attacks of acute pancreatitis were studied. Endotoxaemia, as measured by the limulus lysate assay, was present in 13 of the attacks. Six out of seven patients with systemic complications of the disease had endotoxaemia. C3 catabolism was increased in all 26 attacks of pancreatitis, and a falling level of C3 during attacks of severe pancreatitis was associ...

This unit describes the procedure used to move portions of the E. coli genome from one genetic variant to another. Fragments of approximately 100 kb can be transferred by the P1 bacteriophage. The phage is first grown on a strain containing the elements to be moved, and the resulting phage lysate is used to infect a second recipient strain. The lysate will contain bacterial DNA as well as phage...

Human platelet lysate was shown to contain growth-promoting activity for four well-established malignant cell lines. Platelet lysate was able to support their cell proliferation without plasma or serum, indicating that platelets contained "survival factor" as well as mitogenic factor for these tumor cells. The growth-promoting activity was nondialyzable, heat stable up to 56 degrees, and partia...

(Cell, Vol. 123, 569–580; November 18, 2005) We, the editors of Cell, were contacted by a concerned reader regarding duplication in Figure 2 above paper. Dr. John Blenis, corresponding author, determined that error was introduced during revision manuscript. In preparing final version 2B, panel showing results for eIF3b mislabeled as HA (S6K1) lysate. A different exposure panels published Figure...

ARTICLES DISCUSSED: Grasemann, L., Lavickova, B., Elizondo-Cantú, M. C., Maerkl, S. J. OnePot PURE cell-free system. Journal of Visualized Experiments. (172), doi:10.3791/62625 (2021). Smith, P. E. J., Slouka, T., Dabbas, M., Oza, From cells to protein synthesis within 24 hours using autoinduction workflow. (173), doi:10.3791/62866 Cooper, R. Tonooka, Didovyk, A., Hasty, Rapid, affordable, and ...

In this article we describe the use of the rabbit reticulocyte lysate as an assay system for added mRNA. Some of the techniques we describe are familiar, but we have recently realised that the system can be used in rather subtile ways to provide additional information about the RNA under study. Although our own studies with viral RNA are in their early stages, we believe that the approaches we ...

K. Gambashidze1, I. Pantsulaia2, M. Iobadze2, T. Azaladze1, A. Azaladze1, K. Kalandarishvili3, P. Khorava4, E. Jaiani5, B. Lasareishvili5, M. Tediashvili5 Pathophysiology, Tbilisi State Medical University, Tbilisi, GEORGIA Immunology, Tbilisi State Medical University, Tbilisi, GEORGIA Clinical Anatomy, Tbilisi State Medical University, Tbilisi, GEORGIA Surgery, Georgian National Cancer Center, ...