Extreme environments, generally characterized by atypical temperatures, pH, pressure, salinity, toxicity, and radiation levels, are inhabited by various microorganisms specifically adapted to these particular conditions, called extremophiles. Among these, the microorganisms belonging to the Archaea domain are of significant biotechnological importance as their biopolymers possess unique propert...

Background The plastics derived from petrochemicals have many applications. However, public concern and environmental laws led to conservation policies and establish programs that stimulate the research and use of new products based on renewable resources. Polyhydroxyalkanoates (PHAs) are biopolymers synthesized by microorganisms under conditions of nutrient stress with similar properties to co...

Escherichia coli hosts expressing fabG of Pseudomonas aeruginosa showed 3-ketoacyl coenzyme A (CoA) reductase activity toward R-3-hydroxyoctanoyl-CoA. Furthermore, E. coli recombinants carrying the poly-3-hydroxyalkanoate (PHA) polymerase-encoding gene phaC in addition to fabG accumulated medium-chain-length PHAs (mcl-PHAs) from alkanoates. When E. coli fadB or fadA mutants, which are deficient...

Polyhydroxyalkanoates (PHAs) is reported as polyesters of hydroxyalkanoates (HAs) which is generally synthesized by numerous bacteria and it is stored in the cytoplasm of cells as an intracellular carbon and energy sources compound. It has been considered as a replacement for conventional plastics. Microbial cell such as Alcaligenes, Pseudomonas, recombinant Escherichia coli and methylotrophs a...

The effects on spontaneous ovulation associated with the unilateral or bilateral sectioning of the superior ovarian nerves (SON) were analyzed in guinea pigs at different time intervals of the estrous cycle. Day 1 of the estrous cycle was defined as the day when the animal presents complete loss of the vaginal membrane (open vagina). Subsequent phases of the cycle were determined by counting th...

BACKGROUND The effects of gonadotrophin-releasing hormone agonist (GnRH-a) administered in the luteal phase remains controversial. This meta-analysis aimed to evaluate the effect of the administration of a single-dose of GnRH-a in the luteal phase on ICSI clinical outcomes. METHODS The research strategy included the online search of databases. Only randomized studies were included. The outcom...

We examined the immunohistochemical localization of luteinizing hormone (LH)/human chorionic gonadotrophin (HCG) receptor (LH-R) in the human ovary using the anti-human LH-R monoclonal antibody, 3B5. In the antral follicles, LH-R was detected on theca interna cells. In pre-ovulatory follicles, granulosa cells also expressed LH-R. During corpus luteum formation, granulosa cells seemed to increas...

In the domestic species, regression of the corpus luteum is dependent on uterine release of prostaglandin (PG)F2 alpha. Despite the central role of PGF2 alpha in luteolysis, very little is known about the actual mechanism of prostaglandin-induced regression at the level of the luteal cell. Many studies have focused on the cellular site of action of PGF2 alpha, and it seems likely that this comp...

One of the many roles of tumor necrosis factor (TNF)-α is to control mammalian corpus luteum (CL) PG synthesis and apoptotic cell death. Here, the cellular localization of TNF-α and its type I (TNF-RI) and type II (TNF-RII) receptors in bovine luteal tissue were analyzed using in situ hybridization, immunohistochemistry, and quantitative real-time PCR. Transcripts for TNF-α were expressed in bo...

Polyhydroxyalkanoates (PHAs) have become important due to their mechanical properties, which resemble those of plastics derived from petrochemicals. Despite the advantages they possess, its use is limited by production costs. In terms culture systems used for process improvement, fed batch are most studied, since allow higher productivity be obtained. this study, PHAs was reviewed with a new fe...