Purpose: To investigate the effect of microRNA-612 (miR-612) on human colon cancer cells, and mechanism involved.
 Methods: Expressions miR-612 GPR132 were determined by quantitative real-time polymerase chain reaction (qRT-PCR)el , while cell viability was evaluated using counting kit-8 (CCK8) colony formation assays. Dual luciferase assay used to determine interaction between GPR1...

Ebola virus is currently causing an unprecedented outbreak in West Af-rica. The pathogen is classified as a biosafety level 4 (BSL-4) 1 pathogen and should be handled only in high-level biosafety settings (1). Virus neutralization is considered to be the gold standard serological assay for infections, but in the case of Ebola, this test requires culturing of live virus in a BSL-4 facility. Beca...

Purpose: Ischemia-reperfusion (I/R) injury exacerbates myocardial cell death (including apoptosis and necrosis), leading to complications such as arrhythmias, stenosis, microvascular obstruction heart failure, it is particularly important seek new strategies mitigate reperfusion injury. In this paper, we will investigate whether atorvastatin can alleviate ischemia-reperfusion verify its molecul...


 Purpose: To examine the role of miR-557 in pathogenesis and development glioblastoma (GBM) its potential regulatory mechanism.
 Methods: Dysregulation GBM cells was determined using quantitative (real-time) PCR (qPCR). Cell proliferative ability, migratory activity invasive ability were measured MTT colorimetric assay, wound healing assay Transwell chambers assay. The direct target ...

To investigate the effects and potential mechanisms of chicoric acid (CA) on LPS-induced inflammatory response in A549 cells. 0–800 μM CA was added to cells determine toxicity using MTT assay. Then, 2 μg/mL LPS 50 were simultaneously CA. In order miR-130a-3p IGF-1 cells, infected with inhibitor si RNA IGF-1. The levels cytokines such as IL-1β, IL-6, TNF-α measured by real-time RT-PCR enzyme-lin...

Objective: To explore the role of miR-448 in regulating MAGEA6/AMPK signaling pathway biological study hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using database, related expression chips were obtained and regulatory mirnas candidate genes predicted, predicted results analyzed. The effects MAGEA6 on pellet formation rate clone cells detected by immunofluorescence identification ce...

Abstract Background and Aims Peritoneal dialysis (PD) as a long-term renal replacement therapy is limited by peritoneal fibrosis. The purpose of our study was to identify the mechanism mesenchymal stem cell-derived exosomes (MSC-Exos) in regulating transdifferentiation fibroblasts (FBs) myofibroblasts (MFBs) dialysis-associated Method experimental mice were divided into 3 groups according treat...

Cisplatin represents an important strategy for gastric cancer treatment. However, cisplatin resistance often leads to failure of therapy as well local reoccurrence. Recently, long non-coding ribonucleic acid was demonstrated exert roles in cancer. Expression measured by real time polymerase chain reaction assay cells from different groups. Cell apoptosis checked with Hoechst methods and cell co...

Previous studies have shown that polycystic ovary syndrome (PCOS) patients suffer from low-grade chronic inflammation. Furthermore, our previous confirmed IL-18 is highly expressed in the serum and endometria of PCOS patients. Nevertheless, mechanisms underlying elevation remain unclear. This study aims to explore signaling pathways lead upregulation Using TF-Search tool, we predicted C/EBP-β m...

The aim of this study was to develop a highly sensitive human papillomavirus type 31 (HPV31) neutralization assay based on the production of pseudovirions carrying luciferase. Neutralizing antibodies against HPV31 were investigated in a set of HPV31 monoclonal antibodies and in women with evidence of HPV31 infection. Neutralizing antibodies were detected in 78% of subjects with a positive enzym...