Current techniques for identifying fetal hemoglobin (HbF) inducers are complex and time consuming. We developed a rapid and efficient method for detecting HbF inducers. Our system uses a recombinant DNA construct in which the coding sequences of 2 different luciferase reporter genes, firefly and renilla, are substituted for those of human gamma and beta globin genes, respectively. The activity ...

The effects of cyclic AMP (cAMP)-elevating agents on the activity of cis-acting gene promoter sequences are frequently studied using the luciferase-reporter-gene system. The aim of the present study was to assess whether cAMP-elevating agents induce any changes in the level of luciferase activity independently of a transcriptional activation of promoter elements. Chloramphenicol acteyltransfera...

Transl Androl Urol, Vol 3, Suppl 1 September 2014 www.amepc.org/tau © Translational Andrology and Urology. All rights reserved. effective promoter component can help us overcome these limitations. Methods: Here, we transfected the artificially hTERT promoter sequence into cancer cells and kept the specificity of the promoter. Then we used the Dual luciferase reporter gene system to prove the ar...

13 Yphantis, D. A., Biochemistry, 3, 297 (1964). We are indebted to Dr. Vern Schumaker for the determination of the molecular weights by this method. 14 Arnaud, C., C. Anast, and A. Tenenhouse, in preparation. 15 Rasmussen, H., C. Arnaud, and C. Hawker, Science, 144, 1019 (1964). 16 Moore, S., personal communication. We are indebted to Dr. Moore for several helpful discussions during the course...

The firefly luciferase (fLuc) has been widely utilized for optical reporter gene imaging in biochemical assays, cell culture and living animals. fLuc can catalyze the oxidation of its substrate D-luciferin in the presence of O2, ATP, and Mg 2+ to generate the bioluminescence that can be imaged by a CCD camera. The luciferase based bioluminescence imaging technique displays low background and hi...

Firefly luciferase forms the basis of a wide range of analytical techniques. However, the enzyme is unstable and rapidly loses activity even at room temperature. This leads to losses in sensitivity and precision in analytical applications and also severely limits the fieldability of devices incorporating luciferase-based technologies. A number of point mutations have previously been identified ...

Liu et al. Journal of Microbiologucal Methods, 51, 369-377, 2002.* Ley et al. Can J Microbiol. 39, 821-825, 1993.* Kono, Advanced Drug Delivery Reviews, 53 307-319, 2001.* “Alteration of Tail Fiber Protein gp38 Enables T2 Phage to Infect Escherichia coli 0157:H7” By: Masatoshi Yoichi, Michiham Abe, Kazuhiko Miyanaga, Hajime Unno, Yasunori Tanji, 2005. “Genome Plasticity in the Distal Tail Fiber...

Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such smaller size stronger luminescence activity. We compared the reporter of Nluc Fluc in rice (Oryza sativa). In both plant-based two-hybr...

To monitor the expression of T-DNA-tagged plant genes in vivo, a collection of 20,261 transgenic lines of Arabidopsis (Columbia-0) were generated with the promoter trap vector pTluc, which carries a promoterless firefly luc (luciferase) reporter gene linked to the right T-DNA border. By detection of bioluminescence in 3-week-old seedlings, 753 lines were identified showing constitutive, organ-s...