Extensive spreading of liquid manure onto agricultural fields causes eutrophication of ground and surface water and also pollution of the atmosphere due to the high ammonium nitrogen content. A poly(gamma-glutamic acid) (PGA)-producing strain of Bacillus licheniformis was isolated in this study and investigated for its ability to reduce the ammonium nitrogen by converting ammonium into biomass ...

The cryopreservation of cultured and uncultured amniotic fluid cells is of special importance in prenatal diagnosis. The freezing procedure presented gave a cell recovery of about 95% for cultivated human amniotic fluid cells combined with a rapid appearance of mitosis after inoculation.

Molecular rotors with an off-center axis and the chiral feature of achiral CuPc molecules on a semi-metallic Bi(111) surface have been investigated by means of a scanning tunneling microscopy (STM) at liquid nitrogen (LN₂) temperature. The rotation axis of each CuPc molecular rotor is located at the end of a phthalocyanine group. As molecular coverage increases, the CuPc molecules are self-asse...

Vertical stacks of electrically confined quantum wires were demonstrated in devices with large areas. Multiple current plateaus and strong differential conductance oscillations were observed at above liquid nitrogen temperatures because of interlevel cascade transition of carriers. Our simulation results for charge transport, as well as interlevel infrared photoresponse red-shift, due to latera...

We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of cryo-immersion objectives, current applications of fluorescence cryo-microscopy to biological specimens achieve resolutions between 400-500 nm onl...

Studies of sweet potato (Ipomioca batatas. Poir) mitochondria were extended, in our present investigation, to include spectrophotometric observation of cytochromes in response to substrates and inhibitors. These studies were facilitated by the use of a white variety of sweet potato (var. Pelican Processor which is low in carotenoids, and by the use of a nodlified Perkin-Elmer Spectracord. Cytoc...

In a series of five tests, liquid polyphosphate fertilizer solution was used to treat liquid dairy and swine manure to control odors. Potassium permanganate, a pesticide-wetting agent, nitrogen-zinc liquid fertilizer solution and a digestive deodorant were also used in several of the tests. All tests were evaluated by an odor panel for odor strength and odor quality. The polyphosphates did not ...

UK Biobank required a cryogenic facility to store, in vapour phase, a minimum of 5 000 000 1.2 ml capacity micro-tubes held in racks of 96 tubes below -180 degrees C for a 20 year period. The archive would be housed in a 900 m(2) industrial unit remote from the main laboratory area that would be generally unmanned and would require a complete cryogenic infrastructure. A commercial supplier was ...

Laboratory water window cryomicroscopy has recently demonstrated similar image quality as synchrotron-based microscopy but still with much longer exposure times, prohibiting the spread to a wider scientific community. Here we demonstrate high-resolution laboratory water window imaging of cryofrozen cells with 10 s range exposure times. The major improvement is the operation of a λ=2.48 nm, 2 kH...

We have designed and tested an in-plane echelle spectrograph configured to investigate precision radial velocities from ground-based near-infrared observations. The spectrograph operates across the spectral range of 0.9–1.7 μm at a spectral resolution of R 1⁄4 50; 000, and uses a liquid nitrogen-cooled HAWAII 1 K detector. Repeated measurements of the Earth’s rotation via integrated sunlight wi...