The solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two-dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commerciall...

This research addresses the extraction and recovery of nickel ions from real electroplating wastewater using SLM process. The process involves three main phase system which are feed, organic and stripping phase. The feed phase containing the nickel electroplating wastewater whereas the organic phase containing the liquid membrane which was immobilized in the membrane support. The liquid membran...

In the development of non-radioactive receptor assays for benzodiazepines, employing fluorescent ligands, it was observed that the fluorescence measurements were hampered by the background fluorescence of the receptor preparation. This receptor preparation is a brain tissue homogenate in which the benzodiazepine receptors are membrane-bound. To minimize the influence of the receptor material on...

As a result of genetic and immunological evidence, it is now well established that the induction of murine cytolytic T cells (CTL) depends upon the recognition of antigens which are gene products of the major histocompatibility complex (MHC) of the stimulating cell. A more detailed understanding of the molecular events involved in this specific recognition would be greatly aided by the ability ...

A statistical-mechanical treatment of the solubilization in micelle is presented in combination with molecular simulation. The micellar solution is viewed as an inhomogeneous and partially finite, mixed solvent system, and the method of energy representation is employed to evaluate the free-energy change for insertion of a solute into the micelle inside with a realistic set of potential functio...

Contemporary interest in the enzyme phospholipase A2 (PLA2) understandingly is centred around its role in the release of unsaturated fatty acids as the precursors for other biologically active molecules such as the prostaglandins. However, the other products of the reaction, the lysophosphatides, and in particular lysophosphatidylcholine (LPC), are potent detergents which at high concentrations...