AIM To construct the superantigen SEA (D227A) expression vector modulated by cis-acting element of AFP and express it specifically on AFP(+) hepatocellular carcinoma (HCC) cells. METHODS The enhancer and promoter of AFP gene, SEA (D227A) cDNA and linker-CD80tm were amplified by PCR. The gene segments mentioned above were cloned into the multiple cloning sites of retrovirus vector pLXSN to con...

BACKGROUND The objective of this research was to design an effective gene delivery system composed of cationic solid lipid nanoparticles (SLNs), protamine, and Deoxyribonucleic acid DNA. METHODS Cationic SLNs were prepared using an aqueous solvent diffusion method with octadecylamine as the cationic lipid material. First, protamine was combined with DNA to form binary protamine/DNA nanopartic...

Chronic inflammation, increased reactivity to self-antigens and incidences of cancer are hallmarks of aging. However, the underlying mechanisms are not well understood. Age-associated alterations in the DNA either due to oxidative damage, defects in DNA repair or epigenetic modifications such as methylation that lead to mutations and changes in the expression of genes are thought to be partiall...

BACKGROUND Gemini-lipid nanoparticles have been received major attention recently as non-viral delivery systems due to their successful non-invasive gene delivery through tough barriers such as eye and skin. The aim of this study was to evaluate non-viral gene delivery by a series of dicationic gemini surfactant-phospholipid nanoparticles (GL-NPs) and to explore their mechanism of interaction w...

Abstract Introduction Ischaemia reperfusion injury (IRI) is an unavoidable, significant consequence of renal transplantation. MicroRNAs are small, non-coding RNA molecules that regulate multiple downstream mRNA targets. MiRNA-21-5p and miRNA-24-3p have been previously implicated in IRI. Antisense oligonucleotides (ASOs) block specific microRNAs, with previous work by our group demonstrating the...

Abstract The presence of subcutaneous adipose tissue in excess on a carcass can significantly contribute to financial losses for the producer and processor. Technology with potential limit this accumulation could provide another avenue improve animal efficiency. Stearoyl-CoA desaturase 1 (SCD1) has been identified as genetic target, it seems tohave crucial role fatty acid metabolism. MiR-199a-3...

Gene transfer to mesenchymal stem cells (MSCs) has arisen as a powerful approach increase the therapeutic potential of this effective cell population. Over recent years, niosomes have emerged self-assembled carriers with promising performance for gene delivery. The aim our work was develop niosomes-based DNA delivery platforms targeting MSCs. Niosomes based on 1,2-di-O-octadecenyl-3-trimethylam...

objective: creb1 is an important downstream protein for many signaling pathways. by designing efficient sirnas against creb1, it may be possible to assess the role of molecules involved in signaling pathways in different cell types. in this research the efficiency of creb1 knockdown by two different sirnas in k562 cells has been studied. materials and methods: sirnas have been designed accordi...