A lipid transfer protein, purified from bovine brain (23.7 kDa, 208 amino acids) and specific for glycolipids, has been used to develop a fluorescence resonance energy transfer assay (anthrylvinyl-labeled lipids; energy donors and perylenoyl-labeled lipids; energy acceptors) for monitoring the transfer of lipids between membranes. Small unilamellar vesicles composed of 1 mol% anthrylvinyl-galac...

The antibiotic tunicamycin was separated into 16 different components using reversed-phase high performance liquid chromatography. The effect of the eight major tunicamycin homologues on protein glycosylation and protein biosynthesis was examined. All homologues tested inhibited lipid-mediated protein glycosylation in chick or mouse fibroblasts. These homologues also inhibited the transfer of N...

Background: Phthalates are a group of multifunctional chemicals. Diethyl phthalate (DEP) is one of the most frequently used phthalates in solvents and fixatives for numerous industrial products. Method: The present experiment was designed to assess oxidative stress, if any, caused by diethyl phthalate. For this the homogenates of liver and kidney were treated with different concentrations ( 1...

BACKGROUND Plant food allergies associated with lipid transfer protein (LTP) have been widely described in the Mediterranean Basin. OBJECTIVE The aim of this work was to describe the clinical profile and pollen sensitization of plant food- allergic patients sensitized to LTP in a non-Mediterranean area. METHODS Patients with clear IgE-mediated symptoms associated with plant foods and a posi...

Background Lipid transfer proteins (LTP) are considered true food allergens because sensitization takes place in the gastrointestinal tract, in contrast with other food allergens like profilins or Bet v 1-homologues, where sensitization is a consequence of an allergic sensitization to inhaled allergens. Although allergy to peach LTP (pru p 3) is frequently associated to sensitization to plane t...

Just as non-lipidologists were becoming reconciled to the complexities of networks of ceramide metabolism and the diverse roles of ceramide in cell regulation and membrane structure, a new study reveals a new layer of regulation of ceramide through the actions of a ceramide binding/transfer protein.

background: peroxisome proliferative-activated receptors (ppars) are nuclear receptors that involved in cellular lipid metabolism and differentiation. the subtype ? of the ppar family (ppar?) plays important roles in physiologic functions of ovaries. objective: to determine correlation between ppar? protein level in granulosa cells and pregnancy rate in women undergoing in-vitro fertilization (...

Cafestol and kahweol-diterpenes present in unfiltered coffee-strongly raise serum VLDL and LDL cholesterol and slightly reduce HDL cholesterol in humans. The mechanism of action is unknown. We determined whether the coffee diterpenes may affect lipoprotein metabolism via effects on lipid transfer proteins and lecithin:cholesterol acyltransferase in a randomized, double-blind cross-over study wi...