The development of reliable assay systems that can measure lymphocyte activation in vitro has been a major goal of immunodiagnostics. Traditionally, tritiated thymidine incorporation has been used to monitor T-cell activation. Other methods include enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot assay, and colorimetric assays. We have established a lymphocyte activation assa...

conclusions our results suggest that human exposure to mcpyv occurs throughout life. the mcpyv antibody levels remained high among older adults in our population, consistent with reports from other populations. patients and methods in this cross-sectional study, we collected 440 serum samples from healthy individuals 2 to 78 years of age who visited the pasteur institute’s clinic in tehran, ira...

background: retroviruses of human t-lymphotropic viruses (htlv-1 and htlv-2) have been demonstrated to be endemic in the north-eastern region of iran. this study aims to determine htlv-1 and htlv-2 prevalence among healthy individuals in neyshabur city during 2010-2014. methods: a total of 8054 blood samples were collected from healthy participants in neyshabur, north-eastern iran. the blood sa...

results 34 (34%) of the renal transplant recipients and 26 (26%) of the control group were positive for anti-toxoplasma igg. in addition 18 (18%) of the renal transplant recipients and 4 (4%) of the control group were positive for anti-toxoplasma igm. for both values of the antibodies, differences were statistically significant (p < 0.025 and p < 0.001 respectively). the pcr results indicated t...

conclusions the present study supported the contamination with t. gondii as a risk factor for schizophrenia just in women. results the analyses confirmed the significant differences between healthy women and ones with schizophrenia (p = 0.001) as well as between males and females with schizophrenia (p = 0.009) in igg positivity. materials and methods t. gondii igg antibodies of 100 patients wit...

background there are different types of aflatoxins, including aflatoxin m1, m2, g1, g2, b1 and b2 produced in the food chain, especially when the food is infected with fungi and can directly be consumed by humans. considering that our country is located in the subtropical geographic region where temperature and humidity ecological factors are among determining factors in contamination with afm1...

Metastatic melanoma patients who were treated with patient-specific vaccines consisting of dendritic cells loaded with autologous tumor cells had a 5-year survival of over 50%. Enzyme-linked immunospot (ELISPOT) has been used to detect antigen reactive T cells as a means of determining immune response. We wished to determine whether IFN-gamma secretion in an ELISPOT assay was prognostic or pred...

background: pan-igg specific monoclonal antibodies (mabs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. in this study, four hybridoma clones producing mabs with different specificities for human igg isotypes were established. methods: splenocytes from balb/c mice immunized with fc fractions of human igg were fused with sp2/0 myeloma cells. hybridoma c...

universal vaccination of neonates and children against diphtheria, tetanus and pertussis has had a tremendous impact on the control of these infectious diseases worldwide. immunization by the triple diphtheria, tetanus and whole cell pertussis vaccine (dtwp) has been applied in iran for almost 50 years. periodic assessment of immunogenicity of this vaccine is an important aspect of successful m...

cystic echinococcosis is an important zoonosis in the sheep rising areas of iran. to develop a simple and reliable diagnostic method for echinococcus infection in definitive hosts, e. granulosus polyoclonal antibodies (polyabs) were prepared from adult worm in rabbit. a selected poab was used for coproantigen detection in faecal samples obtained from animals naturally infected with echinococcus...