نتایج جستجو برای: linked enzyme aggregates

تعداد نتایج: 469071  

Journal: :The Journal of biological chemistry 1994
A Puig H F Gilbert

Reduced, denatured lysozyme tends to aggregate at neutral pH, and competition between productive folding and aggregation substantially reduces the efficiency of refolding (Goldberg, M.E., Rudolph, R., and Jaenicke, R. (1991) Biochemistry 30, 2790-2797). Protein disulfide isomerase (PDI), a catalyst of oxidative protein folding, has a variety of effects on the yield of native lysozyme during the...

Journal: :Arthritis Research & Therapy 2008
Sanna Rosengren Nathan Wei Kenneth C Kalunian Nathan J Zvaifler Arthur Kavanaugh David L Boyle

INTRODUCTION The purpose of this study was to quantitatively evaluate the contribution of synovial lymphoid aggregates to autoantibody (rheumatoid factor [RF] and anti-cyclic citrullinated peptide [anti-CCP]) and total immunoglobulin (IgG and IgM) production in rheumatoid arthritis (RA) patients and the effect thereon of the B-cell-depleting antibody, rituximab, in the ARISE (Assessment of Ritu...

Journal: :Langmuir : the ACS journal of surfaces and colloids 2004
Simon Biggs Steven R Kline Lynn M Walker

Solutions of rodlike polymeric micellar aggregates, formed from the polymerization of cetyltrimethyl-ammonium 4-vinylbenzoate (CTVB), adsorb at the solid-liquid interface. The poly-CTVB aggregates are imaged in situ using soft contact atomic force microscopy. The aggregates form self-organized two-dimensional films that show a high degree of order on nanometer to micrometer length scales. Unlik...

Journal: :iranian biomedical journal 0
بهرخ فرهمند behrokh farahmand محمد جواد رسایی mohamad javad rasaee ناصر ملک نیا naser maleknia محمد ملکانه mohamad malekaneh

an enzyme-linked immunosorbent assay (elisa) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. a c3 and c11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (bsa). polyclonal antibody against these two immunogens were prepared in new zealand white rabbits. purified ig fractions of antibodies were immobilized o...

2017
Andrew Care Kerstin Petroll Emily S Y Gibson Peter L Bergquist Anwar Sunna

BACKGROUND Solid-binding peptides (SBPs) bind strongly to a diverse range of solid materials without the need for any chemical reactions. They have been used mainly for the functionalisation of nanomaterials but little is known about their use for the immobilisation of thermostable enzymes and their feasibility in industrial-scale biocatalysis. RESULTS A silica-binding SBP sequence was fused ...

2010
K. Ziervogel C. Arnosti

Marine snow aggregates are heavily colonized by heterotrophic microorganisms that express high levels of hydrolytic activities, making aggregates hotspots for carbon remineralization in the ocean. To assess how aggregate formation influences the ability of seawater microbial communities to access organic carbon, we compared hydrolysis rates of six polysaccharides in coastal seawater after aggre...

Journal: :archives of clinical infectious diseases 0
seyedeh mahya marashiyan department of basic sciences, sari agricultural sciences and natural resources university, sari, iran fatemeh moradian department of basic sciences, sari agricultural sciences and natural resources university, sari, iran geita saadatnia department of biotechnology, iranian research organization for science and technology (irost), tehran, iran; department of biotechnology, iranian research organization for science and technology (irost), tehran, iran. tel/fax: +98-2156276636 majid golkar molecular parasitology laboratory, department of parasitology, pasteur institute of iran, tehran, iran

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

Journal: :Frontiers in catalysis 2022

Genetically encoded photosensitizers are able to produce reactive oxygen species upon illumination and exploited in a wide range of applications, especially the medical field. In this work, we envisioned further apply these genetically for light-dependent control single enzymes multi-step biocatalysis. One challenges application several cascade is unwanted cross-reactivity biocatalysts on react...

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