Activated protein C (APC) is an anticoagulant protease that initiates cell signaling via protease-activated receptor 1 (PAR1) to regulate vascular integrity and inflammatory response. In this study, a recombinant APC variant (APC(N329Q)) mimicking the naturally occurring APC-β plasma glycoform was found to exhibit superior PAR1 proteolysis at a cleavage site that selectively mediates cytoprotec...

Pure phenylalanine hydroxylase from rat liver can be activated by limited proteolysis with alpha-chymotrypsin. As with most other types of activation of this enzyme, including activation by exposure to lysolecithin, the increase in activity is expressed in the presence of the naturally occurring pterin cofactor, tetrahydrobiopterin, but not in the presence of synthetic pterin cofactors such as ...

The interactions of three subunits of cytoplasmic dynein from Drosophila melanogaster, LC8, Tctex-1, and the N-terminal domain of IC74 (N-IC74, residues 1-289), were characterized in vitro by affinity methods, limited proteolysis, and circular dichroism spectroscopy. These subunits were chosen for study because they are presumed to promote the assembly of the complex and to be engaged in the co...

Ataxin-3 is the protein responsible for the neurodegenerative polyglutamine disease Spinocerebellar ataxia type 3. Full structural characterisation of ataxin-3 is required to aid in understanding the mechanism of disease. Despite extensive study, little is known about the conformational properties of the full-length protein, in either its non-expanded healthy or expanded pathogenic forms, parti...

Many enzymes, hormones, and other physiologically active proteins are synthesized as inactive precursors (zymogens) that are subsequently converted to the active form by the selective enzymatic cleavage (limited proteolysis) of peptide bonds. The ultimate agency of activating enzymatic function is limited proteolysis, either in a single activation step or in a consecutive series (cascade). The ...

background and objectives: in this study, the proteolysis and lipolysis of poosti cheese produced from raw sheep milk in mountainous eastern regions of iran were investigated during 90 days of ripening. materials and methods: sodium dodecyl sulfate polyacrylamide gel electrophoresis for proteolysis (sds-page) and gas chromatography (gc) for free fatty acids (ffas) were applied to investigate th...

PURPOSE The aspartyl (Asp) residues 58 and 151 in αA-crystallin, and Asp36 and Asp62 in αB-crystallin in human lenses are known to be highly isomerized with aging. We investigate structural environments of these isomerizable aspartyl residues in α-crystallins of human lenses. METHODS To perform limited proteolysis experiments of purified human αA- and αB-crystallins, endoproteinase Asp-N (EC ...