patients and methods in total, 98 samples in four groups (hiv-positive and hiv-negative persons with and without oc) were obtained from the oral cavities and cultured on sabouraud’s dextrose agar and chromagar. also blood samples were obtained to assess plasma level of ifn-γ, il-4, il-6 and il-17 using elisa technique. background cell-mediated immunity (cmi) by cd4 + th (t helper)-type cells is...

Abstract Background While interferon-lambdas (IFN-λs) were initially discovered for their role in antiviral immunity at mucosal barriers such as the lung and gut, there are many unanswered questions how IFN-λs uniquely dampen inflammatory immune responses. In mouse colitis models, shown to play a significant promoting epithelial barrier integrity healing. Microbes present gut naturally induce I...

In vivo responses to interferon (IFN) in mice were determined by measuring the steady-state levels of induced mRNAs following injection of IFN and poly(I)-poly(C). With cDNA probes for mouse 2'-5' oligoadenylate synthetase (2-5A synthetase) and 1-8, constitutive expression of the corresponding mRNA was detectable in different organs of normal C3H/He mice. These mRNA levels were increased by as ...

Abstract The mechanisms that drive pathology in infection-induced shock are not well understood. tick-borne pathogen Ixodes ovatuserhlichia (IOE) induces severe inflammation, vascular damage, liver injury, and death. Mice lack the receptor for type I IFN, or receptors both II IFNs, improves survival by 50–70 90%, respectively. IOE infection induced a significant increase circulating granulocyte...

Imiquimod has been identified as a potent antiviral and antitumor agent in animal models. The biological activity associated with imiquimod has been attributed to its induction of interferon (IFN)-alpha. The present studies evaluated imiquimod administered orally for its ability to stimulate production of IFN and other cytokines in mice. The cytokine profile induced by imiquimod was compared wi...

Background: Generation of an effective dendritic cell (DC) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. Objective: To compare the effects of monocyte separation methods, flask adherence (Flask-DC) and magnetic activated cell sorting (MACS-DC), on phenotypic and functional characteristics of resultant DCs. Methods: DCs from healthy volunteers were generated ...

abstract pentavalent antimonials, as the 1st choice for the treatment of cutaneous leishmaniasis (cl), have various side effects. also, there are some reports of drug resistance. due to its safety, cryotherapy can be a good alternative or complementary treatment in cl. the aim of this study was to explore the possible systemic immunological mechanisms of cryotherapy besides its local effects in...

BACKGROUND AND AIMS Increased levels of chemokine interferon-gamma (IFN-γ)-inducible protein-10 (CXCL10), soluble CD163 (sCD163) and soluble CD14 (sCD14) have been reported in HCV infection. The aim of this study was to compare, sCD163 and sCD14 levels in HCV-infected patients undergoing direct acting antiviral (DAA)-containing regimens with or without interferon (IFN). METHODS sCD163, sCD14 ...