objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

Gene therapy is a novel method under investigation for the treatment of genetic, metabolic and neurologic diseases, cancer and AIDS. The primary goal of gene therapy is to deliver a specific gene to a pre-determined target cell, and to direct expression of such a gene in a manner which will result in a therapeutic effect. Retroviral vectors have the ability to integrate in the host cell DNA irr...

Studies of lentiviral infections of various animals and man have shown that all may invade the CNS and induce pathological lesions. This is well established in infections with VV, CAEV, SIV, HIV-1, and FIV. Although VV and CAEV do not cause an overt immunodeficiency, they share several features pertinent for the establishment of neuropathologic lesions with those that induce immunodeficiency. T...

The HIV Databases at LANL have primarily focussed on primate lentiviral amino acid and genetic primary sequence information. There is increasing interest in the secondary and tertiary structures of lentiviral proteins as more 3D structures become available. The tools for viewing and manipulating these structures are also becoming more powerful and easier to use. Discovery of the 3D structures o...

Vectors derived from retroviruses, such as MLV-based gammaretroviral vectors, have been used to introduce therapeutic genes into target cells in various clinical gene transfer applications (Hu and Pathak 2000). The stable integration of the vector provides the theoretical potential for long term therapeutic gene expression and may offer persistent beneficial clinical effects in treated patients...