To make a reliable diagnosis on haematological examination, it is necessary to examine further the morphology of blood cells previously stained in procedure for staining thin smears using buffer solution with standard pH 6.4 6.8. One problems that may occur laboratory reagents are damaged, past expiration date or running out, so alternative buffers needed cheap, fast and easy obtain. This study...

BACKGROUND In an endemic area, cutaneous leishmaniasis (CL) is largely diagnosed by its clinical appearance. Diagnostic challenge arises when the lesions appear in nonendemic area, when clinical picture is distorted, or any atypical variant is seen even in endemic regious. In developing countries like ours, the laboratory aid is not widely available and dermatologists mostly have to rely on cli...

Background and Objective: Fine needle aspiration cytology (FNAC) is well accepted as a useful diagnostic technique in the management of adult patients with head and neck lumps. But, until recently, very few reports have been obtained regarding the role of FNAC in nonthyroidal neck masses in children. Hence, the objective of our study was to determine the diagnostic value of fine needle aspirati...

Paraphenylenediamine (PPD) is a synthetic chemical widely used as substitute for henna permanent hair dye because it gives natural look to the and dyed can also be shampooed or permed without losing colour. However, intermediate partially oxidized form of PPD reported cause adverse reactions in people. Effects sublethal concentrations zebra fish were examined 15 days following exposure chemical...

It is well established that a proportion of patients with tobacco amblyopia have pernicious anaemia or some other demonstrable defect of vitamin B12 metabolism (Leishman, 195I; Heaton, McCormick, and Freeman, I958; Freeman and Heaton, I96I; Foulds, Chisholm, Bronte-Stewart, and Wilson, I968). We have examined 65 cases of tobacco amblyopia diagnosed according to the criteria of Heaton and others...

was an important factor in interpreting the results of this test. In the first few months of the disease, Leishman-Donovan bodies may be found in enormous numbers from spleen or liver puncture, although the aldehyde test is negative. During my investigation on this subject, I have found that in various diseases other than kalaazar the blood serum may be changed in such a way that the aldehyde t...

INTRODUCTION Silicification in angiosperms is a phenomenon that has attracted increasing attention in recent years. It is now widely acknowledged that silicification has many benefits to angiosperms (Richmond and Sussman, 2003; Ma and Yamaji, 2008; Epstein, 2009; Guntzer et al., 2012), and that it probably plays appreciable roles at the ecosystem and landscape levels as well (Cooke and Leishman...

In the present study, we investigated the influence of cytological stains in analyzing DNA extracted from cytological slides by comparative genomic hybridization (CGH). Multiple imprint cytological slides were prepared for fresh-frozen breast cancer tissue samples and the slides were stained by three staining methods for each sample. Under microscopic observation, cancer cells were selectively ...

The clinical diagnosis of TRIC-agent infection of the conjunctiva is not difficult for ophthalmologists in endemic areas such as Egypt, but the similarity of the disease to other types of follicular conjunctivitis makes laboratory investigations especially important (Thygeson, I960; Duke-Elder, I965). These include the staining of conjunctival scrapings with Lugol's iodine or Giemsa stain for d...

In order to study the normal hematopoiesis, cellular components and myeloid/erythroid (M/E) ratio in the bone marrow of the pheasant (Phasianus colchicus), bone marrow samples were collected from the proximal tibiotarsus bone of 16 clinically healthy adult pheasant. The bone marrow smears were stained using the Giemsa stain. The results indicated that the development and formation of b...