fertilizer, and manure. Thirty-one strains of Legionella were recovered (table 1). L. longbeachae serogroup 1 was isolated from 8 samples; L. bozemanii serogroup 1, 9; Legionella micdadei, 5; Legionella pneumophila serogroup 4, 1; and L. pneumophila serogroup 12, 1. An unidentifiable Legionella species was isolated from four soil samples; Legionella gormanii, Legionella birminghamensis, and Leg...

The survival of a strain of Legionella pneumophila (Lp-1) inoculated in artificial water microcosms was investigated with and without an amoebal host and varying environmental conditions, such as biofilm formation, amount of nutrients and incubation temperature. The results obtained using short (micromethod) and long (macromethod) term methods showed that L. pneumophila Lp-1 dies rapidly at 4 d...

OBJECTIVES Legionella species are facultative intracellular bacteria. Evaluation of the activity of antibiotics against intracellular L. pneumophila is more predictive of their in vivo efficacy than MICs as determined in axenic medium. However, current methodologies are based on cfu count determination, and are tedious because of the slow growth of Legionella spp. We investigated antibiotic sus...

BACKGROUND Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. It is highly adapted to intracellular replication and manipulates host cell functions like vesicle trafficking and mRNA translation to its own advantage. However, it is still unknown to what extent microRNAs (miRNAs) are involved in the Legionella-host cell interaction. METHODS WT and MyD88-/- murine ...

Legionella pneumophila is a ubiquitous environmental bacterium that thrives in fresh water habitats, either as planktonic form or as part of biofilms. The bacteria also grow intracellularly in free-living protozoa as well as in mammalian alveolar macrophages, thus triggering a potentially fatal pneumonia called "Legionnaires' disease." To establish its intracellular niche termed the "Legionella...

Legionella pneumophila is responsible for hospital or community-acquired pneumonia. Neonatal legionellosis is associated with rapidly severe clinical course and high mortality rates. We describe a case of hospital-acquired Legionella pneumonia in a newborn with undiagnosed tracheoesophageal fistula and acute respiratory failure requiring venovenous extracorporeal membrane oxygenation support be...

Sir, Legionella species are responsible for 1%– 5% of cases of community-acquired pneumonia. Legionella pneumophila serogroup 1 (SG1) accounts for .90% of Legionnaires’ disease (LD) in North America and Europe and is the cause of significant mortality. The mortality rate among patients with L. pneumophila infections continues to be high, up to 26%. The antimicrobial agents most commonly used fo...

introduction: legionnair's disease bacterium is a gram negative, facultative intracellular bacilli which parasitize human alveolar macropages as well as some species of free living amobae. this study was achieved in order to compare the cell wall composition in intracellular bacteria. whole cell and outer membrane proteins and the bacterial lipopolysacharide (lps) were analyzec on sds page syst...

INTRODUCTION Unlike other Legionella species, Legionella longbeachae has been associated with soil and potting composts instead of water systems, and it has caused pneumonia in gardeners. CASE PRESENTATION We report, to our knowledge, the first case of prolonged localized L. longbeachae infection in an accidental wound on the back of a hand caused by a broken flowerpot. CONCLUSION Identific...

Distilled water suspensions of Legionella pneumophila were found to be sensitive to low doses of germicidal ultraviolet radiation.