Matrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping. To this end, four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria. Results suggest that mass spectrometry of intact bacteria could, in some extent, be used to complement the classical morphol...

We previously reported (Sarfare, S., Ahmad, S. T., Joyce, M. V., Boggess, B., and O'Tousa, J. E. (2005) J. Biol. Chem. 280, 11895-11901) that the Drosophila ninaG gene encodes an oxidoreductase involved in the biosynthesis of the (3S)-3-hydroxyretinal serving as chromophore for Rh1 rhodopsin and that ninaG mutant flies expressing Rh4 as the major opsin accumulate large amounts of a different re...

An internal standard method was previously developed to measure the concentration of a synthetic bitter peptide, beta-CN f193-209, by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The objective of this study was to evaluate the relationship between beta-CN f193-209 concentration in an aqueous extract of aged Cheddar cheese and bitterness intensity of ...

This paper reports 16 chemical reactions for elaborating the structures of self-assembled monolayers (SAMs) of alkanethiolates on gold. This work takes advantage of matrix-assisted laser desorption/ionization and time-of-flight mass spectrometry (MALDI-TOF MS) to rapidly characterize the products and yields of reactions that occur with molecules attached to monolayers. The paper also describes ...

For the first time we demonstrate a multiwavelength, ultrabroadband, continuous-wave, tunable, polycrystalline Cr2+:ZnSe spatially-dispersive laser, operating in the near IR spectral region. We show a dual-wavelength operation of the laser, tunable over a 600 nm spectral range (2200-2800 nm), an ultrabroadband operation of the laser, producing a continuous 135 nm wide spectrum centered at 2500 ...

We present a novel technique to generate a continuous, combless broadband Terahertz spectrum with conventional low-cost laser diodes. A standard time-domain spectroscopy system using photoconductive antennas is pumped by the output of two tunable diode lasers. Using fine tuning for one laser and fine and coarse tuning for the second laser, difference frequency generation results in a continuous...

We calculate the quantum-limited shape of the comb lines from a mode-locked Ti:sapphire laser using experimentally-derived parameters for the linear response of the laser to perturbations. The free-running width of the comb lines is found across the laser spectrum. By modeling the effect of a simple feedback loop, we calculate the spectrum of the residual phase noise in terms of the quantum noi...

Semiconductor nanowires (NWs) are fundamental structures for nanoscale devices. The excitation of NWs with laser beams results in thermal effects that can substantially change the spectral shape of the spectroscopic data. In particular, the interpretation of the Raman spectrum is greatly influenced by excitation induced temperature. A study of the interaction of the NWs with the excitation lase...