Introduction:The prevalence of atopic diseases has increased in recent decades dramatically. The most common aeroallergens in Northeastern Iran have not been fully defined. Define the most common aeroallergens in allergic patients based on the skin prick test (SPT) was aimed in this investigation.Materials and Methods:This cross-sectional study enrolled 1,006 allergic patients (aged 1–86 years)...

Oral lactose-ureide is resistant to human digestive enzymes, but is fermented by the colonic microflora. Nine normal adults consuming a diet which provided 36 g of protein/day were given oral doses of lactose-[(13)C]ureide and lactose-[(15)N,(15)N]ureide. The appearance on breath of (13)CO(2) derived from lactose-[(13)C]ureide was followed for 48 h. The fate of (15)N derived from lactose-[(15)N...

A Southern hybridization analysis revealed that the region homologous to Escherichia coli lacZ was present on the chromosomal DNAs of beta-galactosidase-positive Shigella strains, such as Shigella dysenteriae serovar 1 and Shigella sonnei strains, whereas this region was absent from chromosomal DNAs of beta-galactosidase-negative strains of Shigella flexneri and Shigella boydii. We found that t...

BACKGROUND 2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in human milk which protects against the infection of enteric pathogens. Because 2-FL can be synthesized through the enzymatic fucosylation of lactose with guanosine 5'-diphosphate (GDP)-l-fucose by α-1,2-fucosyltransferase (FucT2), an 2-FL producing Escherichia coli can be constructed through overexpressing genes codin...

The construction of Saccharomyces cerevisiae strains that ferment lactose has biotechnological interest, particularly for cheese whey fermentation. A flocculent lactose-consuming S. cerevisiae recombinant expressing the LAC12 (lactose permease) and LAC4 (beta-galactosidase) genes of Kluyveromyces lactis was constructed previously but showed poor efficiency in lactose fermentation. This strain w...

The objective of this study was to determine the effect of different levels of sesame wastes (SW) on performance, milk composition and blood metabolites in lactating dairy cows. In this order, eight multiparous Holstein dairy cows were used in a replicated 4×4 Latin square design in four periods of 21 days. Treatments were control (no Sesame meal supplementat...

The objective of this study was to determine the effect of different levels of sesame wastes (SW) on performance, milk composition and blood metabolites in lactating dairy cows. In this order, eight multiparous Holstein dairy cows were used in a replicated 4×4 Latin square design in four periods of 21 days. Treatments were control (no Sesame meal supplementat...

the effect of different levels of osmolality and ph of lactose extender on the viability of bactrian camelspermatozoa was investigated. semen was collected from 10 bactrian camel bulls using modified bovineartificial vagina. in experiment i, the extenders consisted of 9, 10, 11, 12 and 13% lactose with respectiveosmolalities of 290, 333, 350, 376 and 419 mosm/kg and the ph of 6.9. in experiment...

The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined. The lacE gene encodes the lactose-specific Enzyme II component of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The lacG gene encodes the phospho-beta-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS. T...

[14C]Lactose electroinjected into isolated rat hepatocytes is normally autophagocytosed, transferred to lysosomes and degraded by lysosomal beta-galactosidase, but at high concentrations of asparagine the transfer is inhibited and lactose accumulates in prelysosomal autophagic/endocytic vacuoles (amphisomes). The accumulation can be prevented by addition of yeast beta-galactosidase, which is tr...