Although proteins populate large structural ensembles, X-ray diffraction data are traditionally interpreted using a single model. To search for evidence of alternate conformers, we developed a program, Ringer, which systematically samples electron density around the dihedral angles of protein side chains. In a diverse set of 402 structures, Ringer identified weak, nonrandom electron-density fea...

Homogenates of salivary glands from Locusta migratoria possess phenoloxidase (PO) activity. This study investigates the activation of prophenoloxidase (PPO) in these glands in vitro. When freshly dissected salivary glands from L. migratoria are incubated with the immunogen laminarin, and then homogenized, a 4-fold increase in PO activity (expressed as a percentage of the total PO) can be measur...

Aims: Currently, the blue light of resin curing unit is frequently used in dental treatment. However, the influence of the blue light irradiation on oral tissue is not clear. The aim of this study was to elucidate the effect of dental blue light irradiation on vascular smooth muscle. Methods: Isolated rat descending aorta was suspended in a superfusion chamber and superfused continuously with K...

Culture media for mouse preimplantation development are usually derived from two basic solutions: Krebs-Ringer and Tyrode. We have used outbred mice (OF1) to make a comparative analysis of derived media of both types and their success in sustaining development from the one-cell to the blastocyst stage. The best results (up to 50%), in terms of overcoming the two-cell block and sustaining develo...

Uterine lavage is commonly used in the peri-ovulatory period to improve pregnancy rates in mares with reduced fertility. Previous work has shown that uterine lavage with lactated Ringer’s solution performed immediately before insemination does not affect the fertility in reproductively healthy mares. In the present field study, uterine lavage using lactated Ringer’s solution was performed on a ...

We studied 90 patients undergoing elective Caesarean section under spinal anaesthesia who received lactated Ringer's solution 1000 ml with up to 1000 ml of modified gelatin, lactated Ringer's solution 1000 ml with up to 1000 ml of 6% hydroxyethylstarch or only up to 1000 ml of 6% hydroxyethylstarch. Lumbar puncture was performed as soon as 500 ml of the colloid were infused. The incidence of hy...

A probe consisting of a cotton thread fitted inside a 15-cm Teflon tube with an inner diameter of 0.8 mm and connected to a transducer was used to measure the interstitial pressure in the subcutaneous tissue in six anesthetized dogs. The measurements were reproducible with a standard deviation of ±0.64 mm Hg. In six dogs the mean pressure in the subcutaneous tissue of the thorax was —2.74 mm Hg...

Amaç: Kardiyopulmoner Bypass (KPB) sırasında prime solüsyonu olarak kullanılan farklı sıvıların oksidatif stresi nasıl etkilediğini ortaya koyarak açık kalp cerrahisine katkı sağlamayı amaçlıyoruz. Yöntem: Bu çalışmaya çeşitli nedenlerle ameliyatı geçiren 21 erkek ve 9 kadın olmak üzere toplam 30 hasta dahil edildi. Hastalar solüsyonlarına göre 1. Grup: Laktatlı Ringer, 2 Isolyte S iki gruba ay...