The industrial and environmental applications of laccase, especially in wastewater treatment, have gained focus recent years. Therefore, developing the proper laccase immobilization techniques, which could improve stability enzymes simplify required downstream processes, is needed. A novel two-step process was developed, resulting cross-linked enzyme aggregates (CLEA) pores membrane. Laccase ad...

Abstract Micropollutants are persistent and hazardous materials in low concentrations (ng L −1 –μg ), including substances such as pharmaceuticals, personal care products industrial chemicals. The advancement of analytical chemistry has allowed for the detection micropollutants; however, an efficient economical treatment solution is yet to be installed. Fungal laccase been a successful biocatal...

A bacteria strain Bacillus thuringiensis RUN1identifiedby 16s RNA gene phylogenetic analysiswas used to decolorize malachite green, a triphenylmethane dye in simulated wastewater. The ability of the organism produce extracellular laccase and degrade were also investigated. Results showed that decolorized (84.67 ± 1.19 %) green at 40 mgl-1 within 6 h; decolorization was associated production by ...

Herbicide resistance can be either target-site or non-target-site based. The molecular mechanisms underlying non-target-site resistance (NTSR) are poorly understood, especially at the level of gene expression regulation. MicroRNAs (miRNAs) represent key post-transcriptional regulators of eukaryotic gene expression and play important roles in stress responses. In this study, the miR397 gene from...

Penicillin X methyl ester was transformed into three types of dimer by laccase from Coriolus versicolor. The dimers are considered to be formed by free-radical addition of phenoxy radicals produced by laccase. The enzyme reaction with the ester as substrate was more suitable for forming dimers than that with the sodium salt as substrate. Penicillin X pivaloyloxymethyl ester was also transformed...

Mycelial culture of Pleurotus florida produced highest extracellular laccase in optimum growth medium. At least two laccases (L(inf1) and L(inf2)) were shown to be present in the culture filtrate. Low-laccase-yielding mutants with impaired L(inf2) activity had poor mycelial growth and could not form fruit body, whereas the revertants from the same mutants were similar to the parent in mycelial ...

Photobiocatalysis is an interesting approach to use light perform specific chemical transformations in a selective and efficient way. The intention couple photoredox cycle with enzyme performing multielectronic catalytic activity. Laccase, robust multicopper oxidase, can be envisioned as tool dioxygen clean electron sink when coupled oxidation photocatalyst. Here, we provide detailed study of t...

Nuclear diamination of p-hydrobenzoquinones with aromatic and aliphatic primary amines was catalysed by an immobilised commercial laccase, Denilite II Base, from Novozymes. The amine and the p-hydrobenzoquinone was reacted under mild conditions (at room temperature and at 35 degrees C) in a reaction vessel open to air in the presence of laccase and a co-solvent to afford, exclusively, the diami...

Phenoloxidase activity in crayfish haemocyte lysates and extracts of haemocyte membranes were studied using native PAGE and SDS-PAGE gels and staining for cresolase, catecholase and laccase activities. The activation of the proenzyme, prophenoloxidase to phenoloxidase, in native PAGE was demonstrated following exposure to SDS. By staining samples separated in SDS-PAGE followed by renaturation, ...

In order to study the effect of external ethylene on postharvest display and activity of some senescence-related enzymes in potted miniature roses, this experiment was carried out in a completely randomized design, using three treatment levels with three replications. In glass chambers, potted miniature rose plants were exposed to ethylene concentrations of 0, 5 and 10 μL L-1 for 12 h. The resu...